Product nameAnti-Diphtheria Toxin antibody [IGX3492]
See all Diphtheria Toxin primary antibodies
DescriptionHuman monoclonal [IGX3492] to Diphtheria Toxin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Other species
Recombinant full length protein. This information is considered to be commercially sensitive.
- WB: Diphtheria Toxin (mutated G52 E) full length protein
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant antibody.
Human monoclonal antibody.
For recommended secondary antibodies -
Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 488) - ab200622
Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 647) - ab200623
Rabbit monoclonal Anti-Human IgG1 H&L (Biotin) - ab201485
Rabbit Anti-Human IgG H&L (HRP) - ab6759
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituents: 0.484% Tris buffer, 0.45% Glycine, 40% Glycerol, 1.044% Sodium chloride
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab209329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).|
RelevanceCorynebacterium diphtheriae is a gram-positive, nonmotile bacteria found in soil and animal feces. C. diphteriae infect the epithelial cells of the upper respiratory tract from where they produce and secrete a potent toxin. This toxin is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body.
- Corynebacterium diphtheriae toxoid antibody
- Diphtheria Toxin antibody
- tox antibody
Anti-Diphtheria Toxin antibody [IGX3492] (ab209329) at 1 µg/ml +
Native Diphtheria Toxin (mutated G52 E) protein (ab188505) at 0.1 µg
HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Exposure time: 5 seconds
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209329 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab209329 has not yet been referenced specifically in any publications.