Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Human monoclonal [IGX3492] to Diphtheria Toxin
- Suitable for: WB
Product nameAnti-Diphtheria Toxin antibody [IGX3492]
See all Diphtheria Toxin primary antibodies
DescriptionHuman monoclonal [IGX3492] to Diphtheria Toxin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Other species
Recombinant full length protein. This information is considered to be commercially sensitive.
- WB: Diphtheria Toxin (mutated G52 E) full length protein
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant antibody.
Human monoclonal antibody.
For recommended secondary antibodies -
Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 488) - ab200622
Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 647) - ab200623
Rabbit monoclonal Anti-Human IgG1 H&L (Biotin) - ab201485
Rabbit Anti-Human IgG H&L (HRP) - ab6759
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 40% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab209329 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).|
RelevanceCorynebacterium diphtheriae is a gram-positive, nonmotile bacteria found in soil and animal feces. C. diphteriae infect the epithelial cells of the upper respiratory tract from where they produce and secrete a potent toxin. This toxin is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body.
- Corynebacterium diphtheriae toxoid antibody
- Diphtheria Toxin antibody
- tox antibody
Anti-Diphtheria Toxin antibody [IGX3492] (ab209329) at 1 µg/ml +
Native Diphtheria Toxin (mutated G52E) protein (ab188505) at 0.1 µg
HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Exposure time: 5 seconds
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209329 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab209329 has not yet been referenced specifically in any publications.