Recombinant

Recombinant Anti-Diphtheria Toxin antibody [IGX3492] (ab209329)

Overview

  • Product name

    Anti-Diphtheria Toxin antibody [IGX3492]
    See all Diphtheria Toxin primary antibodies
  • Description

    Human monoclonal [IGX3492] to Diphtheria Toxin
  • Host species

    Human
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Diphtheria Toxin (mutated G52 E) full length protein
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant antibody. 
    Human monoclonal antibody.

    For recommended secondary antibodies -

    Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 488) - ab200622
    Rabbit monoclonal Anti-Human IgG1 H&L (Alexa Fluor® 647) - ab200623
    Rabbit monoclonal Anti-Human IgG1 H&L (Biotin) - ab201485
    Rabbit Anti-Human IgG H&L (HRP)  - ab6759

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Constituents: 0.484% Tris buffer, 0.45% Glycine, 40% Glycerol, 1.044% Sodium chloride
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    IGX3492
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab209329 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).

Target

  • Relevance

    Corynebacterium diphtheriae is a gram-positive, nonmotile bacteria found in soil and animal feces. C. diphteriae infect the epithelial cells of the upper respiratory tract from where they produce and secrete a potent toxin. This toxin is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body.
  • Cellular localization

    Secreted
  • Alternative names

    • Corynebacterium diphtheriae toxoid antibody
    • Diphtheria Toxin antibody
    • tox antibody

Images

  • Anti-Diphtheria Toxin antibody [IGX3492] (ab209329) at 1 µg/ml + Native Diphtheria Toxin (mutated G52 E) protein (ab188505) at 0.1 µg

    Secondary
    HRP conjugated Goat Anti-Human IgG (H+L) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa


    Exposure time: 5 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab209329 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.

References

ab209329 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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