Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [IGX3492R-3] to Diphtheria Toxin
- Suitable for: WB
Product nameAnti-Diphtheria Toxin antibody [IGX3492R-3]
See all Diphtheria Toxin primary antibodies
DescriptionRabbit monoclonal [IGX3492R-3] to Diphtheria Toxin
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Other species
Recombinant full length protein. This information is considered to be commercially sensitive.
- WB: Diphtheria Toxin (mutated G52 E) full length protein
This product was made using synthetic libraries and phage display technology.
This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab219834 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).|
RelevanceCorynebacterium diphtheriae is a gram-positive, nonmotile bacteria found in soil and animal feces. C. diphteriae infect the epithelial cells of the upper respiratory tract from where they produce and secrete a potent toxin. This toxin is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body.
- Corynebacterium diphtheriae toxoid antibody
- Diphtheria Toxin antibody
- tox antibody
Anti-Diphtheria Toxin antibody [IGX3492R-3] (ab219834) at 0.5 µg/ml +
Native Diphtheria Toxin (mutated G52E) protein (ab188505) at 0.1 µg
Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 58 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab219834 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
ab219834 has not yet been referenced specifically in any publications.