Recombinant

Recombinant Anti-Diphtheria Toxin antibody [IGX3492R-3] (ab219834)

Overview

  • Product name

    Anti-Diphtheria Toxin antibody [IGX3492R-3]
    See all Diphtheria Toxin primary antibodies
  • Description

    Rabbit monoclonal [IGX3492R-3] to Diphtheria Toxin
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Other species
  • Immunogen

    Recombinant full length protein. This information is considered to be commercially sensitive.

  • Positive control

    • WB: Diphtheria Toxin (mutated G52 E) full length protein
  • General notes

    This product was made using synthetic libraries and phage display technology.

    This antibody is a recombinant chimeric antibody. Rabbit chimeric monoclonal antibody (Human Fab/ Rabbit Fc).

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.02% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    IGX3492R-3
  • Isotype

    IgG1
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab219834 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.5 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 58 kDa).

Target

  • Relevance

    Corynebacterium diphtheriae is a gram-positive, nonmotile bacteria found in soil and animal feces. C. diphteriae infect the epithelial cells of the upper respiratory tract from where they produce and secrete a potent toxin. This toxin is absorbed and disseminated through lymph channels and blood to the susceptible tissues of the body.
  • Cellular localization

    Secreted
  • Alternative names

    • Corynebacterium diphtheriae toxoid antibody
    • Diphtheria Toxin antibody
    • tox antibody

Images

  • Anti-Diphtheria Toxin antibody [IGX3492R-3] (ab219834) at 0.5 µg/ml + Native Diphtheria Toxin (mutated G52 E) protein (ab188505) at 0.1 µg

    Secondary
    Peroxidase AffiniPure Goat Anti-Rabbit IgG (H+L) at 1/50000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 5 seconds


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 3% milk before being incubated with ab219834 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.

References

ab219834 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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