Anti-DIRAS1 antibody - Carboxyterminal end (ab65139)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-DIRAS1 antibody - Carboxyterminal end
See all DIRAS1 primary antibodies -
Description
Rabbit polyclonal to DIRAS1 - Carboxyterminal end -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total DIRAS1 protein. -
Tested applications
Suitable for: ELISA, WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthesized peptide derived from the C-terminal of human DIRAS1
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Positive control
- Extracts from Hela and HepG2 cells
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab65139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ELISA | 1/20000. | |
| WB | 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
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Relevance
The three human RAS genes encode highly related 188 to 189 amino acid proteins, designated H-Ras, N-Ras and K-Ras4A and K-Ras4B (the two K-Ras proteins arise from alternative gene splicing). Ras proteins function as binary molecular switches that control intracellular signaling networks. Ras-regulated signal pathways control such processes as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. Ras and ras-related proteins are often deregulated in cancers, leading to increased invasion and metastasis, and decreased apoptosis. DIRAS1 displays low GTPase activity and exists predominantly in the GTP-bound form. It is highly expressed in heart and brain. -
Cellular localization
Cell membrane; Lipid-anchor; Cytoplasmic side -
Database links
- Entrez Gene: 148252 Human
- Omim: 607862 Human
- SwissProt: O95057 Human
- Unigene: 172753 Human
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Alternative names
- Di Ras1 antibody
- DIRAS family GTP binding RAS like 1 antibody
- Distinct subgroup of the Ras family member 1 antibody
see all
Images
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Western blot - Anti-DIRAS1 antibody - Carboxyterminal end (ab65139)All lanes : Anti-DIRAS1 antibody - Carboxyterminal end (ab65139) at 1/500 dilution
Lane 1 : Extracts from Hela cells
Lane 2 : Extracts from HepG2 cells
Lane 3 : Extracts from HepG2 cells, plus immunising peptide
Predicted band size: 22 kDa
Observed band size: 22 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug.
Protocols
Datasheets and documents
References
ab65139 has not yet been referenced specifically in any publications.
