Anti-DIRAS1 antibody - Carboxyterminal end (ab65139)
Key features and details
- Rabbit polyclonal to DIRAS1 - Carboxyterminal end
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DIRAS1 antibody - Carboxyterminal end
See all DIRAS1 primary antibodies -
Description
Rabbit polyclonal to DIRAS1 - Carboxyterminal end -
Host species
Rabbit -
Specificity
This antibody detects endogenous levels of total DIRAS1 protein. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
A synthesized peptide derived from the C-terminal of human DIRAS1
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Positive control
- Extracts from Hela and HepG2 cells
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab65139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa). |
Target
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Relevance
The three human RAS genes encode highly related 188 to 189 amino acid proteins, designated H-Ras, N-Ras and K-Ras4A and K-Ras4B (the two K-Ras proteins arise from alternative gene splicing). Ras proteins function as binary molecular switches that control intracellular signaling networks. Ras-regulated signal pathways control such processes as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. Ras and ras-related proteins are often deregulated in cancers, leading to increased invasion and metastasis, and decreased apoptosis. DIRAS1 displays low GTPase activity and exists predominantly in the GTP-bound form. It is highly expressed in heart and brain. -
Cellular localization
Cell membrane; Lipid-anchor; Cytoplasmic side -
Database links
- Entrez Gene: 148252 Human
- Omim: 607862 Human
- SwissProt: O95057 Human
- Unigene: 172753 Human
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Alternative names
- Di Ras1 antibody
- DIRAS family GTP binding RAS like 1 antibody
- Distinct subgroup of the Ras family member 1 antibody
see all
Images
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Western blot - Anti-DIRAS1 antibody - Carboxyterminal end (ab65139)All lanes : Anti-DIRAS1 antibody - Carboxyterminal end (ab65139) at 1/500 dilution
Lane 1 : Extracts from Hela cells
Lane 2 : Extracts from HepG2 cells
Lane 3 : Extracts from HepG2 cells, plus immunising peptide
Predicted band size: 22 kDa
Observed band size: 22 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug.
Datasheets and documents
References (0)
ab65139 has not yet been referenced specifically in any publications.
