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Signal Transduction Signaling Pathway G Protein Signaling Small G Proteins Ras Family
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Anti-DIRAS1 antibody - Carboxyterminal end (ab65139)

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Western blot - Anti-DIRAS1 antibody - Carboxyterminal end (ab65139)

    Key features and details

    • Rabbit polyclonal to DIRAS1 - Carboxyterminal end
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

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    Secondary
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    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Protein
    Product image
    Recombinant Human DIRAS1 protein (ab99389)

    View more associated products

    Overview

    • Product name

      Anti-DIRAS1 antibody - Carboxyterminal end
      See all DIRAS1 primary antibodies
    • Description

      Rabbit polyclonal to DIRAS1 - Carboxyterminal end
    • Host species

      Rabbit
    • Specificity

      This antibody detects endogenous levels of total DIRAS1 protein.
    • Tested applications

      Suitable for: WBmore details
    • Species reactivity

      Reacts with: Human
    • Immunogen

      A synthesized peptide derived from the C-terminal of human DIRAS1

    • Positive control

      • Extracts from Hela and HepG2 cells
    • General notes

      Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

      Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

      We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

      In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

      We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

      Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

      Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
    • Storage buffer

      pH: 7.40
      Preservative: 0.02% Sodium azide
      Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride

      Without Mg2+ and Ca2+
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Signal Transduction
      • Signaling Pathway
      • G Protein Signaling
      • Small G Proteins
      • Ras Family
      • Cancer
      • Signal transduction
      • G protein signaling
      • Small G proteins
      • Ras family

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human DIRAS1 protein (ab99389)

    Applications

    Our Abpromise guarantee covers the use of ab65139 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Abreviews Notes
    WB 1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).

    Target

    • Relevance

      The three human RAS genes encode highly related 188 to 189 amino acid proteins, designated H-Ras, N-Ras and K-Ras4A and K-Ras4B (the two K-Ras proteins arise from alternative gene splicing). Ras proteins function as binary molecular switches that control intracellular signaling networks. Ras-regulated signal pathways control such processes as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. Ras and ras-related proteins are often deregulated in cancers, leading to increased invasion and metastasis, and decreased apoptosis. DIRAS1 displays low GTPase activity and exists predominantly in the GTP-bound form. It is highly expressed in heart and brain.
    • Cellular localization

      Cell membrane; Lipid-anchor; Cytoplasmic side
    • Database links

      • Entrez Gene: 148252 Human
      • Omim: 607862 Human
      • SwissProt: O95057 Human
      • Unigene: 172753 Human
      • Alternative names

        • Di Ras1 antibody
        • DIRAS family GTP binding RAS like 1 antibody
        • Distinct subgroup of the Ras family member 1 antibody
        • FLJ42681 antibody
        • GBTS1 antibody
        • GTP binding protein Di Ras1 antibody
        • Ras related inhibitor of cell growth antibody
        • RIG antibody
        • Rig protein antibody
        • Small GTP binding tumor suppressor 1 antibody
        see all

      Images

      • Western blot - Anti-DIRAS1 antibody - Carboxyterminal end (ab65139)
        Western blot - Anti-DIRAS1 antibody - Carboxyterminal end (ab65139)
        All lanes : Anti-DIRAS1 antibody - Carboxyterminal end (ab65139) at 1/500 dilution

        Lane 1 : Extracts from Hela cells
        Lane 2 : Extracts from HepG2 cells
        Lane 3 : Extracts from HepG2 cells, plus immunising peptide

        Predicted band size: 22 kDa
        Observed band size: 22 kDa



        The amount of positive control loading for the WB is 5-30 ug of total protein.
        The amount of the peptide for the WB is 5-10 ug.

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab65139? Please let us know so that we can cite the reference in this datasheet.

      ab65139 has not yet been referenced specifically in any publications.

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