Key features and details
- Rabbit polyclonal to DIRAS1 - Carboxyterminal end
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DIRAS1 antibody - Carboxyterminal end
See all DIRAS1 primary antibodies
DescriptionRabbit polyclonal to DIRAS1 - Carboxyterminal end
SpecificityThis antibody detects endogenous levels of total DIRAS1 protein.
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
A synthesized peptide derived from the C-terminal of human DIRAS1
- Extracts from Hela and HepG2 cells
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine), 0.87% Sodium chloride
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab65139 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 22 kDa (predicted molecular weight: 22 kDa).|
RelevanceThe three human RAS genes encode highly related 188 to 189 amino acid proteins, designated H-Ras, N-Ras and K-Ras4A and K-Ras4B (the two K-Ras proteins arise from alternative gene splicing). Ras proteins function as binary molecular switches that control intracellular signaling networks. Ras-regulated signal pathways control such processes as actin cytoskeletal integrity, proliferation, differentiation, cell adhesion, apoptosis, and cell migration. Ras and ras-related proteins are often deregulated in cancers, leading to increased invasion and metastasis, and decreased apoptosis. DIRAS1 displays low GTPase activity and exists predominantly in the GTP-bound form. It is highly expressed in heart and brain.
Cellular localizationCell membrane; Lipid-anchor; Cytoplasmic side
- Di Ras1 antibody
- DIRAS family GTP binding RAS like 1 antibody
- Distinct subgroup of the Ras family member 1 antibody
All lanes : Anti-DIRAS1 antibody - Carboxyterminal end (ab65139) at 1/500 dilution
Lane 1 : Extracts from Hela cells
Lane 2 : Extracts from HepG2 cells
Lane 3 : Extracts from HepG2 cells, plus immunising peptide
Predicted band size: 22 kDa
Observed band size: 22 kDa
The amount of positive control loading for the WB is 5-30 ug of total protein.The amount of the peptide for the WB is 5-10 ug.
ab65139 has not yet been referenced specifically in any publications.