Anti-DIRAS2 antibody (ab67430)
Key features and details
- Mouse polyclonal to DIRAS2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DIRAS2 antibody -
Description
Mouse polyclonal to DIRAS2 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant full length protein within Human DIRAS2. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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Positive control
- DIRAS2 transfected 293T cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.4
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab67430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa).
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Notes |
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WB
1/500 - 1/1000. Detects a band of approximately 24 kDa (predicted molecular weight: 22 kDa). |
Target
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Relevance
DIRAS2 displays low GTPase activity and exist predominantly in the GTP-bound form. It is highly expressed in the brain. -
Cellular localization
Cell membrane; Lipid-anchor; Cytoplasmic side -
Database links
- Entrez Gene: 54769 Human
- Omim: 607863 Human
- SwissProt: Q96HU8 Human
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Alternative names
- Di Ras2 antibody
- DIRAS family, GTP binding RAS like 2 antibody
- DIRAS2 antibody
see all
Images
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All lanes : Anti-DIRAS2 antibody (ab67430) at 1/500 dilution
Lane 1 : DIRAS2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
Secondary
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 22 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (1)
ab67430 has been referenced in 1 publication.
- Ying K et al. Diverse Ras-related GTPase DIRAS2, downregulated by PSMD2 in a proteasome-mediated way, inhibits colorectal cancer proliferation by blocking NF-κB signaling. Int J Biol Sci 18:1039-1050 (2022). PubMed: 35173535