Product nameAnti-DIRAS2 antibody
DescriptionMouse polyclonal to DIRAS2
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Rat, Human
Full length DIRAS2 protein (Human)
- DIRAS2 transfected 293T cell lysate
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: None
Constituents: PBS, pH 7.2
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab67430 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Detection Antibody Pair.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
RelevanceDIRAS2 displays low GTPase activity and exist predominantly in the GTP-bound form. It is highly expressed in the brain.
Cellular localizationCell membrane; Lipid-anchor; Cytoplasmic side
- Di Ras2 antibody
- DIRAS family, GTP binding RAS like 2 antibody
- DIRAS2 antibody
All lanes : Anti-DIRAS2 antibody (ab67430) at 1/500 dilution
Lane 1 : DIRAS2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg/ml per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size: 22 kDa
Observed band size: 24 kDa why is the actual band size different from the predicted?
ab67430 staining DIRAS2 in rat brain tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde, a heat mediated antigen retrieval step was performed using Tris-EDTA pH9, samples were then blocked using 5% serum and then incubated with ab67430 at a 1/100 dilution for 1 hour at 25°C. The secondary used was an undiluted biotin conjugated goat polyclonal.
ab67430 has not yet been referenced specifically in any publications.