Product nameAnti-DIS antibody
See all DIS primary antibodies
DescriptionRabbit polyclonal to DIS
Tested applicationsSuitable for: WB, IP, IHC-Pmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Chimpanzee, Rhesus monkey, Gorilla, Tilapia, Orangutan
Synthetic peptide corresponding to a region between N terminal residues 1 and 50 of human DIS. (SwissProt Q8IX12)
- Nuclear extract from HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.80
Preservative: 0.09% Sodium azide
Tris-citrate/phosphate buffer, pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab70243 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/15000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).|
|IP||Use at 1-10 µg/mg of lysate.|
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
FunctionAssociates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation.
Tissue specificityExpressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors.
Sequence similaritiesContains 1 SAP domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
- CARP-1 antibody
- Carp1 antibody
- Ccar1 antibody
All lanes : Anti-DIS antibody (ab70243) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lane 4 : TCMK-1 whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 132 kDa
Exposure time: 10 seconds
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma (left) and mouse renal cell carcinoma (right) tissues labelling DIS with ab70243 at 1/1000 (1µg/ml). Detection: DAB.
Immunoprecipitation analysis of whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells (lanes 1 and 2). ab70243 was used for IP at 6 µg/mg lysate. For western blot ab70243 was used at 1 µg/ml. Lane 3 - control IgG.
ab70243 has not yet been referenced specifically in any publications.