Anti-DIS antibody (ab70245)
Key features and details
- Rabbit polyclonal to DIS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DIS antibody
See all DIS primary antibodies -
Description
Rabbit polyclonal to DIS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Elephant -
Immunogen
Region between residue 1100 and the C terminus (residue 1150) of human DIS
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Positive control
- Nuclear extract from HeLa cells
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Tris-citrate/phosphate buffer, pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antibody was affinity purified using an epitope specific to DIS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | ||
IP |
Notes |
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WB: 1/1000 - 1/10000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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Function
Associates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation. -
Tissue specificity
Expressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors. -
Sequence similarities
Contains 1 SAP domain. -
Post-translational
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR. -
Cellular localization
Cytoplasm > perinuclear region. - Information by UniProt
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Database links
- Entrez Gene: 450498 Chimpanzee
- Entrez Gene: 479230 Dog
- Entrez Gene: 55749 Human
- Omim: 612569 Human
- SwissProt: Q8IX12 Human
- Unigene: 49853 Human
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Alternative names
- CARP-1 antibody
- Carp1 antibody
- Ccar1 antibody
see all
Images
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Lanes 1-2 : Anti-DIS antibody (ab70245) at 1 µg/ml
Lanes 3-4 : Anti-DIS antibody (ab70245) at 0.2 µg/ml
Lanes 1 & 3 : Nuclear extract from HeLa cells at 15 µg
Lanes 2 & 4 : Nuclear extract from HeLa cells at 45 µg
Developed using the ECL technique.
Predicted band size: 132 kDa
Observed band size: 132 kDa
Exposure time: 1 minute -
Detection of Human CCAR1 by Immunoprecipitation using Nuclear extract (100 µg) from HeLa cells and Affinity purified rabbit anti-CCAR1 ab70245 at 1 µg/100 µg Nuclear extract. Chemiluminescence with exposure times of approximately 3 seconds.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab70245 has not yet been referenced specifically in any publications.