Key features and details
- Rabbit polyclonal to DIS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DIS antibody
See all DIS primary antibodies
DescriptionRabbit polyclonal to DIS
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Dog, Chimpanzee, Rhesus monkey, Elephant
Region between residue 1100 and the C terminus (residue 1150) of human DIS
- Nuclear extract from HeLa cells
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.80
Preservative: 0.09% Sodium azide
Tris-citrate/phosphate buffer, pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntibody was affinity purified using an epitope specific to DIS immobilized on solid support.
Our Abpromise guarantee covers the use of ab70245 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/1000 - 1/10000. Detects a band of approximately 132 kDa (predicted molecular weight: 132 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
FunctionAssociates with components of the Mediator and p160 coactivator complexes that play a role as intermediaries transducing regulatory signals from upstream transcriptional activator proteins to basal transcription machinery at the core promoter. Recruited to endogenous nuclear receptor target genes in response to the appropriate hormone. Also functions as a p53 coactivator. May thus play an important role in transcriptional regulation (By similarity). May be involved in apoptosis signaling in the presence of the reinoid CD437. Apoptosis induction involves sequestration of 14-3-3 protein(s) and mediated altered expression of multiple cell cycle regulatory genes including MYC, CCNB1 and CDKN1A. Plays a role in cell cycle progression and/or cell proliferation.
Tissue specificityExpressed in various epithelial cancer cell lines, including breast, colon, prostate, pancreatic and leukemia. Expression is regulated by growth factors.
Sequence similaritiesContains 1 SAP domain.
modificationsPhosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localizationCytoplasm > perinuclear region.
- Information by UniProt
- CARP-1 antibody
- Carp1 antibody
- Ccar1 antibody
Lanes 1-2 : Anti-DIS antibody (ab70245) at 1 µg/ml
Lanes 3-4 : Anti-DIS antibody (ab70245) at 0.2 µg/ml
Lanes 1 & 3 : Nuclear extract from HeLa cells at 15 µg
Lanes 2 & 4 : Nuclear extract from HeLa cells at 45 µg
Developed using the ECL technique.
Predicted band size: 132 kDa
Observed band size: 132 kDa
Exposure time: 1 minute
Detection of Human CCAR1 by Immunoprecipitation using Nuclear extract (100 µg) from HeLa cells and Affinity purified rabbit anti-CCAR1 ab70245 at 1 µg/100 µg Nuclear extract. Chemiluminescence with exposure times of approximately 3 seconds.
ab70245 has not yet been referenced specifically in any publications.