Key features and details
- Rabbit polyclonal to dis2
- Suitable for: WB, IP
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG
Product nameAnti-dis2 antibody
DescriptionRabbit polyclonal to dis2
Specificityab122909 is specific to dis2 and does not cross-react with Sds21.
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Schizosaccharomyces pombe
Recombinant full length Schizosaccharomyces pombe dis2.
- S.pombe cell extract.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferpH: 6
Preservative: 0.05% Sodium azide
Constituent: 99% Whole serum
Concentration information loading...
Our Abpromise guarantee covers the use of ab122909 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/300 - 1/1000. Predicted molecular weight: 37 kDa.|
|IP||Use at an assay dependent dilution.|
RelevanceEssential role in cell cycle control. PP1 is perhaps required for exit from mitosis.
- bws1 antibody
- Serine/threonine-protein phosphatase PP1-1 antibody
All lanes : Anti-dis2 antibody (ab122909) at 1/300 dilution
Lane 1 : S.pombe cells wild type
Lane 2 : S.pombe cells dis2 deletion mutant
Predicted band size: 37 kDa
Immunoprecipitation of wild-type S. pombe extracts was performed using ab122909. Resulting supernatant (S) and immunoprecipitate (P) were immunoblotted by anti-dis2 antibody.
ab122909 has been referenced in 3 publications.
- Ishii K et al. Requirement for PP1 phosphatase and 20S cyclosome/APC for the onset of anaphase is lessened by the dosage increase of a novel gene sds23+. EMBO J 15:6629-40 (1996). PubMed: 8978689
- Yamano H et al. Phosphorylation of dis2 protein phosphatase at the C-terminal cdc2 consensus and its potential role in cell cycle regulation. EMBO J 13:5310-8 (1994). PubMed: 7957097
- Stone EM et al. Mitotic regulation of protein phosphatases by the fission yeast sds22 protein. Curr Biol 3:13-26 (1993). PubMed: 15335873