Anti-DIS3 antibody (ab223767)
Key features and details
- Rabbit polyclonal to DIS3
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-DIS3 antibody
See all DIS3 primary antibodies -
Description
Rabbit polyclonal to DIS3 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WB, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment corresponding to Human DIS3 aa 800-950.
Database link: Q9Y2L1 -
Positive control
- IHC-P: Human testis, lymph node, cervix and cerebellum tissue. WB: U-87 MG, NIH/3T3 and NBT-II cell lysates. ICC/IF: U-2 OS cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab223767 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB |
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 109 kDa.
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ICC/IF |
Use a concentration of 0.25 - 2 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
Notes |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
Use a concentration of 0.04 - 0.4 µg/ml. Predicted molecular weight: 109 kDa. |
ICC/IF
Use a concentration of 0.25 - 2 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
Target
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Function
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the RNR ribonuclease family.
Contains 1 PINc domain. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3. - Information by UniProt
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Database links
- Entrez Gene: 22894 Human
- Entrez Gene: 72662 Mouse
- Entrez Gene: 306103 Rat
- Omim: 607533 Human
- SwissProt: Q9Y2L1 Human
- SwissProt: Q9CSH3 Mouse
- Unigene: 728935 Human
- Unigene: 163339 Mouse
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Alternative names
- 2810028N01Rik antibody
- bA555G22 antibody
- Dis3 antibody
see all
Images
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Paraffin-embedded human testis tissue stained for DIS3 using ab223767 at 1/200 dilution in immunohistochemical analysis.
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All lanes : Anti-DIS3 antibody (ab223767) at 1/100 dilution
Lane 1 : NIH/3T3 (mouse embryo fibroblast cell line) cell lysate
Lane 2 : NBT-II cell lysate
Predicted band size: 109 kDa -
PFA-fixed, Triton X-100 permeabilized U-2 OS (human bone osteosarcoma epithelial cell line) cells stained for DIS3 (green) using ab223767 at 4 µg/ml in ICC.
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Paraffin-embedded human lymph node tissue stained for DIS3 using ab223767 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human cervix tissue stained for DIS3 using ab223767 at 1/200 dilution in immunohistochemical analysis.
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Paraffin-embedded human cerebellum tissue stained for DIS3 using ab223767 at 1/200 dilution in immunohistochemical analysis.
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Anti-DIS3 antibody (ab223767) at 1/100 dilution + U-87 MG (human glioblastoma-astrocytoma epithelial cell line) cell lysate
Predicted band size: 109 kDa
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab223767 has not yet been referenced specifically in any publications.