Overview

  • Product name

  • Description

    Rabbit polyclonal to DIS3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rat, Rabbit, Horse, Cow, Dog, Pig, Chimpanzee, Cynomolgus monkey, Rhesus monkey, Orangutan
  • Immunogen

    Synthetic peptide within Human DIS3 aa 1-50. The exact sequence is proprietary. (NP_055768.3).
    Database link: Q9Y2L1

  • Positive control

    • WB: HeLa, HEK-293T and Jurkat whole cell lysates. IP: HeLa whole cell lysate.
  • General notes

     This product was previously labelled as DIS3, Dis3

     

Properties

Applications

Our Abpromise guarantee covers the use of ab226186 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 109 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the RNR ribonuclease family.
    Contains 1 PINc domain.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3.
  • Information by UniProt
  • Database links

  • Alternative names

    • 2810028N01Rik antibody
    • bA555G22 antibody
    • Dis3 antibody
    • DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease antibody
    • DIS3 mitotic control homolog (S. cerevisiae) antibody
    • DIS3 mitotic control homolog antibody
    • DIS3 mitotic control, S. cerevisiae, homolog of antibody
    • dis3p antibody
    • EXOSC11 antibody
    • Exosome complex exonuclease RRP44 antibody
    • exosome component 11 antibody
    • KIAA1008 antibody
    • Mitotic control protein dis3 homolog antibody
    • Protein DIS3 homolog antibody
    • Ribosomal RNA processing protein 44 antibody
    • Ribosomal RNA processing protein 44, S. cerevisiae, homolog of antibody
    • Ribosomal RNA-processing protein 44 antibody
    • RP11-342J4.3 antibody
    • RRP44 antibody
    • RRP44_HUMAN antibody
    see all

Images

  • All lanes : Anti-DIS3 antibody (ab226186) at 0.1 µg/ml

    Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
    Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
    Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg

    Developed using the ECL technique.

    Predicted band size: 109 kDa


    Exposure time: 3 minutes
  • DIS3 was immunoprecipitated from HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226186 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226186 at 1 µg/ml.

    Lane 1: ab226186 IP in HeLa whole cell lysate.

    Lane 2: Control IgG IP in HeLa whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 30 seconds.

References

ab226186 has not yet been referenced specifically in any publications.

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