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    dis3-antibody-ab64289.pdf

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Anti-DIS3 antibody (ab64289)

  • Datasheet
Submit a review Submit a question References (1)

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Immunocytochemistry/ Immunofluorescence - Anti-DIS3 antibody (ab64289)
  • Western blot - Anti-DIS3 antibody (ab64289)

Key features and details

  • Rabbit polyclonal to DIS3
  • Suitable for: WB, ICC/IF
  • Reacts with: Schizosaccharomyces pombe
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-DIS3 antibody
    See all DIS3 primary antibodies
  • Description

    Rabbit polyclonal to DIS3
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Schizosaccharomyces pombe
    WB
    Schizosaccharomyces pombe
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Schizosaccharomyces pombe DIS3.
    Database link: P37202

  • Positive control

    • S. pombe cell lysates transformed with vector or plasmids carrying truncated genes; S.pombe cells.
  • General notes

     This product was previously labelled as DIS3, Dis3

     

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 6
    Preservative: 0.05% Sodium azide
    Constituent: 50% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • DNA / RNA
    • RNA Processing
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Other cell division antibodies
    • Microbiology
    • Organism
    • Eukaryotic Microorganism
    • Yeast
    • Other Yeast species

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab64289 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Schizosaccharomyces pombe
WB
Schizosaccharomyces pombe
Application Abreviews Notes
WB
1/100 - 1/300. Predicted molecular weight: 110 kDa.
ICC/IF
Use at an assay dependent concentration.
Notes
WB
1/100 - 1/300. Predicted molecular weight: 110 kDa.
ICC/IF
Use at an assay dependent concentration.

Target

  • Function

    Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.
  • Tissue specificity

    Widely expressed.
  • Sequence similarities

    Belongs to the RNR ribonuclease family.
    Contains 1 PINc domain.
  • Cellular localization

    Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3.
  • Target information above from: UniProt accession Q9Y2L1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Alternative names

    • 2810028N01Rik antibody
    • bA555G22 antibody
    • Dis3 antibody
    • DIS3 homolog, exosome endoribonuclease and 3'-5' exoribonuclease antibody
    • DIS3 mitotic control homolog (S. cerevisiae) antibody
    • DIS3 mitotic control homolog antibody
    • DIS3 mitotic control, S. cerevisiae, homolog of antibody
    • dis3p antibody
    • EXOSC11 antibody
    • Exosome complex exonuclease RRP44 antibody
    • exosome component 11 antibody
    • KIAA1008 antibody
    • Mitotic control protein dis3 homolog antibody
    • Protein DIS3 homolog antibody
    • Ribosomal RNA processing protein 44 antibody
    • Ribosomal RNA processing protein 44, S. cerevisiae, homolog of antibody
    • Ribosomal RNA-processing protein 44 antibody
    • RP11-342J4.3 antibody
    • RRP44 antibody
    • RRP44_HUMAN antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-DIS3 antibody (ab64289)
    Immunocytochemistry/ Immunofluorescence - Anti-DIS3 antibody (ab64289)

    Immunfluorescent analysis of S. pombe cells labeling DIS with ab64289 (right panel). DAPI stain for chromosomal DNA (left panel).

  • Western blot - Anti-DIS3 antibody (ab64289)
    Western blot - Anti-DIS3 antibody (ab64289)
    All lanes : Anti-DIS3 antibody (ab64289) at 1/100 dilution

    Lane 1 : S. pombe cell lysate transformed with the vector
    Lane 2 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (172)
    Lane 3 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (A)
    Lane 4 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (B)
    Lane 5 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (C)
    Lane 6 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (E)

    Predicted band size: 110 kDa



    Polypeptides of expected molecular masses were detected.

Protocols

  • Western blot protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab64289? Please let us know so that we can cite the reference in this datasheet.

    ab64289 has been referenced in 1 publication.

    • Kinoshita N  et al. The fission yeast dis3+ gene encodes a 110-kDa essential protein implicated in mitotic control. Mol Cell Biol 11:5839-47 (1991). PubMed: 1944266

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