Product nameAnti-DIS3 antibody
See all DIS3 primary antibodies
DescriptionRabbit polyclonal to DIS3
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Schizosaccharomyces pombe
Recombinant fragment corresponding to Schizosaccharomyces pombe DIS3.
Database link: P37202
- S. pombe cell lysates transformed with vector or plasmids carrying truncated genes; S.pombe cells.
This product was previously labelled as DIS3, Dis3
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 50% Glycerol
Concentration information loading...
Our Abpromise guarantee covers the use of ab64289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/100 - 1/300. Predicted molecular weight: 110 kDa.|
|ICC/IF||Use at an assay dependent concentration.|
FunctionPutative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities.
Tissue specificityWidely expressed.
Sequence similaritiesBelongs to the RNR ribonuclease family.
Contains 1 PINc domain.
Cellular localizationCytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3.
- Information by UniProt
- 2810028N01Rik antibody
- bA555G22 antibody
- Dis3 antibody
Immunfluorescent analysis of S. pombe cells labeling DIS with ab64289 (right panel). DAPI stain for chromosomal DNA (left panel).
All lanes : Anti-DIS3 antibody (ab64289) at 1/100 dilution
Lane 1 : S. pombe cell lysate transformed with the vector
Lane 2 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (172)
Lane 3 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (A)
Lane 4 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (B)
Lane 5 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (C)
Lane 6 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (E)
Predicted band size: 110 kDa
Polypeptides of expected molecular masses were detected.