Anti-DIS3 antibody (ab64289)
Key features and details
- Rabbit polyclonal to DIS3
- Suitable for: WB, ICC/IF
- Reacts with: Schizosaccharomyces pombe
- Isotype: IgG
Overview
-
Product name
Anti-DIS3 antibody
See all DIS3 primary antibodies -
Description
Rabbit polyclonal to DIS3 -
Host species
Rabbit -
Tested Applications & Species
See all applications and species dataApplication Species ICC/IF Schizosaccharomyces pombeWB Schizosaccharomyces pombe
-
Immunogen
Recombinant fragment corresponding to Schizosaccharomyces pombe DIS3.
Database link: P37202 -
Positive control
- S. pombe cell lysates transformed with vector or plasmids carrying truncated genes; S.pombe cells.
-
General notes
This product was previously labelled as DIS3, Dis3
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6
Preservative: 0.05% Sodium azide
Constituent: 50% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab64289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| ICC/IF |
Schizosaccharomyces pombe
|
| WB |
Schizosaccharomyces pombe
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/100 - 1/300. Predicted molecular weight: 110 kDa.
|
|
| ICC/IF |
Use at an assay dependent concentration.
|
| Notes |
|---|
|
WB
1/100 - 1/300. Predicted molecular weight: 110 kDa. |
|
ICC/IF
Use at an assay dependent concentration. |
Target
-
Function
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the RNR ribonuclease family.
Contains 1 PINc domain. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3. - Information by UniProt
-
Alternative names
- 2810028N01Rik antibody
- bA555G22 antibody
- Dis3 antibody
see all
Images
-
Immunocytochemistry/ Immunofluorescence - Anti-DIS3 antibody (ab64289)
Immunfluorescent analysis of S. pombe cells labeling DIS with ab64289 (right panel). DAPI stain for chromosomal DNA (left panel).
-
Western blot - Anti-DIS3 antibody (ab64289)All lanes : Anti-DIS3 antibody (ab64289) at 1/100 dilution
Lane 1 : S. pombe cell lysate transformed with the vector
Lane 2 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (172)
Lane 3 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (A)
Lane 4 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (B)
Lane 5 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (C)
Lane 6 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (E)
Predicted band size: 110 kDaPolypeptides of expected molecular masses were detected.
Protocols
Datasheets and documents
References (1)
ab64289 has been referenced in 1 publication.
- Kinoshita N et al. The fission yeast dis3+ gene encodes a 110-kDa essential protein implicated in mitotic control. Mol Cell Biol 11:5839-47 (1991). PubMed: 1944266
