- Datasheet
- Specific References (1)
- Protocols
Overview
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Product nameAnti-Dis3 antibody
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DescriptionRabbit polyclonal to Dis3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IFmore details
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Species reactivityReacts with: Schizosaccharomyces pombe
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Immunogen
Recombinant fragment corresponding to Schizosaccharomyces pombe Dis3.
Database link: P37202 -
Positive control
- S. pombe cell lysates transformed with vector or plasmids carrying truncated genes; S.pombe cells.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.05% Sodium azide
Constituent: 50% Glycerol -
PurityWhole antiserum
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab64289 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/100 - 1/300. Predicted molecular weight: 110 kDa. | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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RelevanceDis 3 is a catalytic component of the exosome 3'->5' exoribonuclease complex required for the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA and for mRNA decay. Has a 3'-5' exonuclease activity. Implicated in mitotic control. Essential for cell division and spore germination. May be involved in regulating protein dephosphorylation during mitosis.
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Cellular localizationCytoplasmic and Nuclear
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Database links
- Entrez Gene: 2540398 Schizosaccharomyces pombe
- SwissProt: P37202 Schizosaccharomyces pombe
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Alternative names
- 3'-5' exoribonuclease subunit Dis3 antibody
- Chromosome disjunction protein 3 antibody
- Exosome complex exonuclease dis3 antibody
see all
Images
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Immunocytochemistry/ Immunofluorescence - Anti-Dis3 antibody (ab64289)Immunfluorescent analysis of S. pombe cells labeling Dis3 with ab64289 (right panel). DAPI stain for chromosomal DNA (left panel).
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Western blot - Anti-Dis3 antibody (ab64289)All lanes : Anti-Dis3 antibody (ab64289) at 1/100 dilution
Lane 1 : S. pombe cell lysate transformed with the vector
Lane 2 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (172)
Lane 3 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (A)
Lane 4 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (B)
Lane 5 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (C)
Lane 6 : S. pombe cell lysate transformed with a plasmid carrying truncated gene (E)
Predicted band size: 110 kDaPolypeptides of expected molecular masses were detected.
References
This product has been referenced in:
- Kinoshita N et al. The fission yeast dis3+ gene encodes a 110-kDa essential protein implicated in mitotic control. Mol Cell Biol 11:5839-47 (1991). Read more (PubMed: 1944266) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"