Anti-DIS3 antibody - C-terminal (ab226204)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-DIS3 antibody - C-terminal
See all DIS3 primary antibodies -
Description
Rabbit polyclonal to DIS3 - C-terminal -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Chimpanzee, Gorilla
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Immunogen
Synthetic peptide within Human DIS3 aa 858-908 (C terminal). The exact sequence is proprietary. NP_055768.3
Database link: Q9Y2L1 -
Positive control
- IP: HEK-293T whole cell lysate. WB: HeLa, HEK-293T and Jurkat whole cell lysate.
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General notes
This product was previously labelled as DIS3, Dis3
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab226204 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Predicted molecular weight: 109 kDa. | |
| IP | Use at 2-10 µg/mg of lysate. |
Target
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Function
Putative catalytic component of the RNA exosome complex which has 3'->5' exoribonuclease activity and participates in a multitude of cellular RNA processing and degradation events. In the nucleus, the RNA exosome complex is involved in proper maturation of stable RNA species such as rRNA, snRNA and snoRNA, in the elimination of RNA processing by-products and non-coding 'pervasive' transcripts, such as anti-sense RNA species and promoter-upstream transcripts (PROMPTs), and of mRNAs with processing defects, thereby limiting or excluding their export to the cytoplasm. The RNA exosome may be involved in Ig class switch recombination (CSR) and/or Ig variable region somatic hypermutation (SHM) by targeting AICDA deamination activity to transcribed dsDNA substrates. In the cytoplasm, the RNA exosome complex is involved in general mRNA turnover and specifically degrades inherently unstable mRNAs containing AU-rich elements (AREs) within their 3' untranslated regions, and in RNA surveillance pathways, preventing translation of aberrant mRNAs. It seems to be involved in degradation of histone mRNA. DIS3 has both 3'-5' exonuclease and endonuclease activities. -
Tissue specificity
Widely expressed. -
Sequence similarities
Belongs to the RNR ribonuclease family.
Contains 1 PINc domain. -
Cellular localization
Cytoplasm. Nucleus > nucleolus. Nucleus > nucleoplasm. Nucleus. Predominantly located in the nucleus. According to PubMed:12429849, found in the nucleolus and according to PubMed:20531386, excluded from nucleolus supporting the existence of a nucleolar RNA exosome complex devoid of DIS3. - Information by UniProt
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Database links
- Entrez Gene: 452595 Chimpanzee
- Entrez Gene: 22894 Human
- Omim: 607533 Human
- SwissProt: Q9Y2L1 Human
- Unigene: 728935 Human
- Unigene: 744104 Human
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Alternative names
- 2810028N01Rik antibody
- bA555G22 antibody
- Dis3 antibody
see all
Images
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Western blot - Anti-DIS3 antibody - C-terminal (ab226204)All lanes : Anti-DIS3 antibody - C-terminal (ab226204) at 0.1 µg/ml
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 15 µg
Lane 3 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Lane 4 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 109 kDa
Exposure time: 30 seconds -
Immunoprecipitation - Anti-DIS3 antibody - C-terminal (ab226204)DIS3 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226204 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226204 at 1 µg/ml.
Lane 1: ab226204 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 10 seconds.
Datasheets and documents
References
ab226204 has not yet been referenced specifically in any publications.
