Product nameAnti-DIS3L2 antibody
See all DIS3L2 primary antibodies
DescriptionRabbit polyclonal to DIS3L2
Tested applicationsSuitable for: IHC-P, WB, ELISAmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Horse, Guinea pig, Cow, Cat, Dog, Pig
- Jurkat Cell Lysate or Human Myocardial cells
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 2% Sucrose, PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab50848 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 4 - 8 µg/ml.|
|WB||Use a concentration of 1.25 µg/ml. Detects a band of approximately 63 kDa (predicted molecular weight: 69 kDa). Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|ELISA||Use at an assay dependent concentration.
ELISA titre using peptide based assay: 1/62500.
FunctionRibonuclease that plays a critical role in RNA metabolism. It is essential for correct mitosis, and negatively regulates cell proliferation.
Involvement in diseaseDefects in DIS3L2 are the cause of Perlman syndrome (PRLMNS) [MIM:267000]. An autosomal recessive congenital overgrowth syndrome. Affected children are large at birth, are hypotonic, and show organomegaly, characteristic facial dysmorphisms (inverted V-shaped upper lip, prominent forehead, deep-set eyes, broad and flat nasal bridge, and low-set ears), renal anomalies (nephromegaly and hydronephrosis), frequent neurodevelopmental delay, and high neonatal mortality. Perlman syndrome is associated with a high risk of Wilms tumor. Histologic examination of the kidneys in affected children shows frequent nephroblastomatosis, which is a precursor lesion for Wilms tumor.
Sequence similaritiesBelongs to the RNR ribonuclease family.
- Information by UniProt
- DI3L2_HUMAN antibody
- DIS3 L2 antibody
- DIS3 mitotic control homolog (S. cerevisiae) like 2 antibody
All lanes : Anti-DIS3L2 antibody (ab50848) at 1.25 µg/ml
Lane 1 : MW marker
Lane 2 : Jurkat cell lysate at 10 µg
Lane 2 : HRP conjugated anti-Rabbit IgG at 1/50000 dilution
Predicted band size: 69 kDa
Observed band size: 63 kDa why is the actual band size different from the predicted?
Gel concentration: 12%.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human heart tissue labelling DIS3L2 with ab50848 at 4-8µg/ml. Arrows indicate positively stained human myocardial cells. Magnification: 400X.
ab50848 has not yet been referenced specifically in any publications.