Overview

  • Product name

  • Description

    Rabbit polyclonal to DISC1
  • Host species

    Rabbit
  • Specificity

    No cross reactivity with other proteins.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide corresponding to Human DISC1 aa 461-477 (internal sequence).
    Sequence:

    EKQQLQKEIEALQARMF


    Database link: Q9NRI5

  • Positive control

    • IHC: Rat cerebellum and human lung cancer tissues; WB: U87 Whole Cell Lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab191455 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 94 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A. Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Plays a role, together with PCNT, in the microtubule network formation.
  • Tissue specificity

    Ubiquitous. Highly expressed in the dentate gyrus of the hippocampus. Also expressed in the temporal and parahippocampal cortices and cells of the white matter.
  • Involvement in disease

    Note=A chromosomal aberration involving DISC1 segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Translocation t(1;11)(q42.1;q14.3). The truncated DISC1 protein produced by this translocation is unable to interact with ATF4, ATF5 and NDEL1.
    Genetic variation in DISC1 is associated with susceptibility to schizophrenia type 9 (SCZD9) [MIM:604906]. A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder.
  • Developmental stage

    Expression rises within the dentate gyrus and temporal cortex from the neonatal period to infancy, declines markedly in adolescence, and declines further with aging.
  • Cellular localization

    Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Colocalizes with NDEL1 in the perinuclear region and the centrosome (By similarity). Localizes to punctate cytoplasmic foci which overlap in part with mitochondria. Colocalizes with PCNT at the centrosome.
  • Information by UniProt
  • Database links

  • Alternative names

    • C1orf136 antibody
    • DISC1 antibody
    • DISC1_HUMAN antibody
    • Disrupted in schizophrenia 1 antibody
    • Disrupted in schizophrenia 1 protein antibody
    • KIAA0457 antibody
    • RP4-730B13.1 antibody
    • SCZD9 antibody
    see all

Images

  • Anti-DISC1 antibody (ab191455) at 0.5 µg/ml + U87 Whole Cell Lysate at 50 µg with 5% Non-fat Milk/ TBS

    Secondary
    goat anti-rabbit IgG-HRP at 1/10000 dilution

    Predicted band size: 94 kDa

  • Immunohistochemical analysis of parafifn embedded human lung cancer tissue staining DISC1 with ab191455 at 1µg/ml. Biotinylated goat anti-rabbit IgG was used as a secondary antibody. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins.

    The tissue section was developed using Strepavidin-Biotin-Complex (SABC), with DAB as the chromogen.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) of rat cerebellum tissue staining DISC1 with ab191455 at 1 μg/ml.

    Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.

References

ab191455 has not yet been referenced specifically in any publications.

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