Recombinant Anti-DISC1 antibody [EPR14684] (ab192258)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR14684] to DISC1
- Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF, IHC-P
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-DISC1 antibody [EPR14684]
See all DISC1 primary antibodies -
Description
Rabbit monoclonal [EPR14684] to DISC1 -
Host species
Rabbit -
Specificity
This antibody has cross-reactivity with GST, so we suggest not to use this antibody for detecting protein with a GST-tag. -
Tested applications
Suitable for: Flow Cyt (Intra), WB, IP, ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Molt-4, SH-SY5Y, U87-MG, C6, RAW264.7, PC12, NIH/3T3 and HeLa whole cell lysate (ab150035). Human brain, mouse brain and human adenocarcinoma of endometrium tissue. Neuro-2a cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR14684 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab192258 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
1/1000.
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WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 94 kDa.
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IP |
1/60.
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ICC/IF |
1/1000.
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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Flow Cyt (Intra)
1/1000. |
WB
1/1000 - 1/10000. Predicted molecular weight: 94 kDa. |
IP
1/60. |
ICC/IF
1/1000. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Involved in the regulation of multiple aspects of embryonic and adult neurogenesis. Required for neural progenitor proliferation in the ventrical/subventrical zone during embryonic brain development and in the adult dentate gyrus of the hippocampus. Participates in the Wnt-mediated neural progenitor proliferation as a positive regulator by modulating GSK3B activity and CTNNB1 abundance. Plays a role as a modulator of the AKT-mTOR signaling pathway controlling the tempo of the process of newborn neurons integration during adult neurogenesis, including neuron positioning, dendritic development and synapse formation. Inhibits the activation of AKT-mTOR signaling upon interaction with CCDC88A. Regulates the migration of early-born granule cell precursors toward the dentate gyrus during the hippocampal development. Plays a role, together with PCNT, in the microtubule network formation. -
Tissue specificity
Ubiquitous. Highly expressed in the dentate gyrus of the hippocampus. Also expressed in the temporal and parahippocampal cortices and cells of the white matter. -
Involvement in disease
Note=A chromosomal aberration involving DISC1 segregates with schizophrenia and related psychiatric disorders in a large Scottish family. Translocation t(1;11)(q42.1;q14.3). The truncated DISC1 protein produced by this translocation is unable to interact with ATF4, ATF5 and NDEL1.
Genetic variation in DISC1 is associated with susceptibility to schizophrenia type 9 (SCZD9) [MIM:604906]. A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. -
Developmental stage
Expression rises within the dentate gyrus and temporal cortex from the neonatal period to infancy, declines markedly in adolescence, and declines further with aging. -
Cellular localization
Cytoplasm. Cytoplasm > cytoskeleton. Cytoplasm > cytoskeleton > centrosome. Cell junction > synapse > postsynaptic cell membrane > postsynaptic density. Colocalizes with NDEL1 in the perinuclear region and the centrosome (By similarity). Localizes to punctate cytoplasmic foci which overlap in part with mitochondria. Colocalizes with PCNT at the centrosome. - Information by UniProt
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Database links
- Entrez Gene: 27185 Human
- Entrez Gene: 244667 Mouse
- Entrez Gene: 307940 Rat
- Omim: 605210 Human
- SwissProt: Q9NRI5 Human
- SwissProt: Q811T9 Mouse
- SwissProt: Q810H6 Rat
- Unigene: 13318 Human
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Alternative names
- C1orf136 antibody
- DISC1 antibody
- DISC1_HUMAN antibody
see all
Images
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All lanes : Anti-DISC1 antibody [EPR14684] (ab192258) at 1/10000 dilution
Lane 1 : MOLT-4 cell lysate
Lane 2 : SH-SY5Y cell lysate
Lane 3 : U87-MG cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 94 kDa -
Immunohistochemical analysis of paraffin embedded mouse brain tissue sections labeling DISC1 using ab192258 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunofluorescent analysis of 4% formaldehyde fixed Neuro-2a cells labeling DISC1 using ab192258 at a 1/100 dilution. A Goat anti rabbit IgG (Alexa Fluor®488) ab150077 was used as the secondary at a 1/200 dilution. Permeabilized using 0.1% Triton X-100. Counterstained with DAPI. The two negative controls: 1. Primary ab concentration (anti-DISC1 ) is 1:1000 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution; 2. Primary ab concentration (anti-DISC1 ) is 1:1000 dilution, Secondary ab (Goat anti mouse IgG (Alexa Fluor®594)) is 1:500 dilution.
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Intracellular Flow Cytometry analysis of Neuro-2a (Mouse neuroblastoma neuroblast) cells labeling DISC1 (red) with purified ab192258 at a 1/1000 dilution (1ug/mL). Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti rabbit IgG (Alexa Fluorr® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).
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Lysate from U87-MG cells was immunoprecipitated with ab192258 at a 1/60 dilution. For the subsequent blot, ab192258 used at a 1/1000 dilution with Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at a 1/1000 dilution for the secondary. Blocking/ Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-DISC1 antibody [EPR14684] (ab192258) at 1/1000 dilution
Lane 1 : C6 cell lysate
Lane 2 : RAW264.7 cell lysate
Lane 3 : PC-12 cell lysate
Lane 4 : NIH/3T3 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 94 kDa -
Immunohistochemical analysis of paraffin embedded human brain tissue sections labeling DISC1 using ab192258 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Immunohistochemical analysis of paraffin embedded Human adenocarcinoma of endometrium tissue sections labeling DISC1 using ab192258 at a 1/500 dilution. A ready to use HRP Polymer for Rabbit IgG was used as the secondary. Hematoxylin counterstain.
Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (4)
ab192258 has been referenced in 4 publications.
- Zhou D et al. GLYX-13 Ameliorates Schizophrenia-Like Phenotype Induced by MK-801 in Mice: Role of Hippocampal NR2B and DISC1. Front Mol Neurosci 11:121 (2018). PubMed: 29695955
- Chen Y et al. HERV-W env regulates calcium influx via activating TRPC3 channel together with depressing DISC1 in human neuroblastoma cells. J Neurovirol N/A:N/A (2018). PubMed: 30397826
- Zhang Q et al. Electrical Stimulation Using Conductive Polymer Polypyrrole Counters Reduced Neurite Outgrowth of Primary Prefrontal Cortical Neurons from NRG1-KO and DISC1-LI Mice. Sci Rep 7:42525 (2017). WB . PubMed: 28198409
- Lin YH et al. Opening a New Time Window for Treatment of Stroke by Targeting HDAC2. J Neurosci 37:6712-6728 (2017). PubMed: 28592694