Anti-Dishevelled / Dvl1 antibody (ab233003)
Key features and details
- Rabbit polyclonal to Dishevelled / Dvl1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dishevelled / Dvl1 antibody -
Description
Rabbit polyclonal to Dishevelled / Dvl1 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Human Dishevelled/ Dvl1 aa 150-300. Two N-terminal Tags; Expressed in E.coli.
Database link: O14640-1 -
Positive control
- WB: Human lung lysate; Recombinant human Dishevelled / Dvl1 protein. IHC-P: Human liver and glioma tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
Antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab233003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 1 - 5 µg/ml.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 1 - 5 µg/ml. |
Target
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Function
Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ). -
Involvement in disease
Robinow syndrome, autosomal dominant 2 -
Sequence similarities
Belongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. -
Domain
The DIX domain promotes homooligomerization.
The DEP domain mediates interaction with the cell membrane. -
Post-translational
modificationsUbiquitinated; undergoes both 'Lys-48'-linked ubiquitination, leading to its subsequent degradation by the ubiquitin-proteasome pathway, and 'Lys-63'-linked ubiquitination. The interaction with INVS is required for ubiquitination. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains. -
Cellular localization
Cell membrane. Cytoplasm, cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members. - Information by UniProt
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Database links
- Entrez Gene: 457191 Chimpanzee
- Entrez Gene: 1855 Human
- Entrez Gene: 13542 Mouse
- Entrez Gene: 83721 Rat
- Omim: 601365 Human
- SwissProt: Q5IS48 Chimpanzee
- SwissProt: O14640 Human
- SwissProt: P51141 Mouse
see all -
Form
There are 2 isoforms produced by alternative splicing. -
Alternative names
- Dishevelled 1 antibody
- dishevelled 1 like antibody
- Dishevelled antibody
see all
Images
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Formalin-fixed, paraffin-embedded human glioma tissue stained for Dishevelled / Dvl1 using ab233003 at 20 ug/ml in immunohistochemical analysis. DAB staining.
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Formalin-fixed, paraffin-embedded human liver tissue stained for Dishevelled / Dvl1 using ab233003 at 20 ug/ml in immunohistochemical analysis. DAB staining.
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Anti-Dishevelled / Dvl1 antibody (ab233003) at 2 µg/ml + Human lung tissue
Secondary
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution -
Anti-Dishevelled / Dvl1 antibody (ab233003) at 2 µg/ml + Recombinant human Dishevelled / Dvl1 protein
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab233003 has been referenced in 8 publications.
- Bukovac A et al. Bilateral Meningioma: A Case Report and Review of the Literature. Int J Mol Sci 23:N/A (2022). PubMed: 35163107
- Pruller J et al. DVL1 and DVL3 require nuclear localisation to regulate proliferation in human myoblasts. Sci Rep 12:8388 (2022). PubMed: 35589804
- Zhang LQ et al. DKK3 ameliorates neuropathic pain via inhibiting ASK-1/JNK/p-38-mediated microglia polarization and neuroinflammation. J Neuroinflammation 19:129 (2022). PubMed: 35658977
- Yin H et al. FUBP1 promotes colorectal cancer stemness and metastasis via DVL1-mediated activation of Wnt/ß-catenin signaling. Mol Oncol N/A:N/A (2021). PubMed: 34288405
- Wang Y et al. Identification of colon tumor marker NKD1 via integrated bioinformatics analysis and experimental validation. Cancer Med 10:7383-7394 (2021). PubMed: 34547189
- Bukovac A et al. Decoding the Role of DVL1 in Intracranial Meningioma. Int J Mol Sci 22:N/A (2021). PubMed: 34769425
- Peng Y & Wang HH Cir-ITCH inhibits gastric cancer migration, invasion and proliferation by regulating the Wnt/ß-catenin pathway. Sci Rep 10:17443 (2020). PubMed: 33060778
- Xu Y et al. Casiopeina II-gly acts on lncRNA MALAT1 by miR-17-5p to inhibit FZD2 expression via the Wnt signaling pathway during the treatment of cervical carcinoma. Oncol Rep N/A:N/A (2019). PubMed: 31524268