Key features and details
- Rabbit polyclonal to Dishevelled / Dvl1
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dishevelled / Dvl1 antibody
DescriptionRabbit polyclonal to Dishevelled / Dvl1
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chimpanzee
Recombinant fragment (His-T7-tag) corresponding to Human Dishevelled/ Dvl1 aa 150-285. Two N-terminal Tags; Expressed in E.coli.
RRNREEAARTNGHPRGDRRRDVGLPPDSASTALSSELESSSFVDSDEDGS TSRLSSSTEQSTSSRLIRKHKRRRRKQRLRQADRASSFSSITDSTMSLNI VTVTLNMERHHFLGISIVGQSNDRGDGGIYIGSIMK
Database link: O14640-1
- WB: Human lung lysate; Recombinant human Dishevelled / Dvl1 protein. IHC-P: Human liver and glioma tissue.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Our Abpromise guarantee covers the use of ab233003 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 1 - 5 µg/ml.|
FunctionParticipates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. Required for LEF1 activation upon WNT1 and WNT3A signaling. DVL1 and PAK1 form a ternary complex with MUSK which is important for MUSK-dependent regulation of AChR clustering during the formation of the neuromuscular junction (NMJ).
Involvement in diseaseRobinow syndrome, autosomal dominant 2
Sequence similaritiesBelongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain.
DomainThe DIX domain promotes homooligomerization.
The DEP domain mediates interaction with the cell membrane.
modificationsUbiquitinated; undergoes both 'Lys-48'-linked ubiquitination, leading to its subsequent degradation by the ubiquitin-proteasome pathway, and 'Lys-63'-linked ubiquitination. The interaction with INVS is required for ubiquitination. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
Cellular localizationCell membrane. Cytoplasm, cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members.
- Information by UniProt
FormThere are 2 isoforms produced by alternative splicing.
- Dishevelled 1 antibody
- dishevelled 1 like antibody
- Dishevelled antibody
Formalin-fixed, paraffin-embedded human glioma tissue stained for Dishevelled / Dvl1 using ab233003 at 20 ug/ml in immunohistochemical analysis. DAB staining.
Formalin-fixed, paraffin-embedded human liver tissue stained for Dishevelled / Dvl1 using ab233003 at 20 ug/ml in immunohistochemical analysis. DAB staining.
Anti-Dishevelled / Dvl1 antibody (ab233003) at 2 µg/ml + Human lung tissue
HRP-Linked Guinea pig anti-rabbit at 1/2000 dilution
Anti-Dishevelled / Dvl1 antibody (ab233003) at 2 µg/ml + Recombinant human Dishevelled / Dvl1 protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab233003 has been referenced in 1 publication.
- Xu Y et al. Casiopeina II-gly acts on lncRNA MALAT1 by miR-17-5p to inhibit FZD2 expression via the Wnt signaling pathway during the treatment of cervical carcinoma. Oncol Rep N/A:N/A (2019). PubMed: 31524268