Overview

  • Product name

    Anti-Dishevelled 2 antibody
    See all Dishevelled 2 primary antibodies
  • Description

    Rabbit polyclonal to Dishevelled 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Monkey
  • Immunogen

    Recombinant fragment, corresponding to a region within internal sequence amino acids 223-550 of Human Dishevelled 2 (NP_004413).

  • Positive control

    • 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji whole cell lysates, Human lung squamous cell carcinoma tissue.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab137528 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 79 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).

Target

  • Function

    Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
  • Sequence similarities

    Belongs to the DSH family.
    Contains 1 DEP domain.
    Contains 1 DIX domain.
    Contains 1 PDZ (DHR) domain.
  • Domain

    The DIX domain mediates homooligomerization.
  • Cellular localization

    Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dishevelled 2 (homologous to Drosophila dsh) antibody
    • Dishevelled dsh homolog 2 antibody
    • dishevelled segment polarity protein 2 antibody
    • Dishevelled-2 antibody
    • Dishevelled2 antibody
    • DSH homolog 2 antibody
    • DVL 2 antibody
    • Dvl2 antibody
    • DVL2_HUMAN antibody
    • Segment polarity protein dishevelled homolog DVL 2 antibody
    • Segment polarity protein dishevelled homolog DVL-2 antibody
    • Segment polarity protein dishevelled homolog DVL2 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin embedded Human lung squamous cell carcinoma tissue labelling Dishevelled 2 with ab137528 at 1/100 dilution.
  • All lanes : Anti-Dishevelled 2 antibody (ab137528) at 1/500 dilution

    Lane 1 : Molt4 whole cell lysate
    Lane 2 : Raji whole cell lysate

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 79 kDa



    7.5% SDS PAGE

References

This product has been referenced in:

  • Wang S  et al. SH2B1 promotes epithelial-mesenchymal transition through the IRS1/ß-catenin signaling axis in lung adenocarcinoma. Mol Carcinog 57:640-652 (2018). WB ; Human . Read more (PubMed: 29380446) »
See 1 Publication for this product

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