Product nameAnti-Dishevelled 2 antibody
See all Dishevelled 2 primary antibodies
DescriptionRabbit polyclonal to Dishevelled 2
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Monkey
Recombinant fragment, corresponding to a region within internal sequence amino acids 223-550 of Human Dishevelled 2 (NP_004413).
- 293T, A431, H1299, HeLaS3, HepG2, Molt-4, Raji whole cell lysates, Human lung squamous cell carcinoma tissue.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
- HepG2 cytoplasmic extract lysate (ab14659)
- Raji whole cell lysate (ab30124)
- Raji membrane extract lysate (ab30126)
- A431 whole cell lysate (ab30132)
- A431 membrane extract lysate (ab30134)
- HepG2 whole cell lysate (ab7900)
- A431 whole cell lysate (ab7909)
- MOLT4 whole cell lysate (ab7912)
- 293T whole cell lysate (ab95494)
Our Abpromise guarantee covers the use of ab137528 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 79 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).|
FunctionParticipates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
Sequence similaritiesBelongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain.
DomainThe DIX domain mediates homooligomerization.
Cellular localizationCell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
- Information by UniProt
- Dishevelled 2 (homologous to Drosophila dsh) antibody
- Dishevelled dsh homolog 2 antibody
- dishevelled segment polarity protein 2 antibody
Immunohistochemical analysis of paraffin embedded Human lung squamous cell carcinoma tissue labelling Dishevelled 2 with ab137528 at 1/100 dilution.
All lanes : Anti-Dishevelled 2 antibody (ab137528) at 1/500 dilution
Lane 1 : Molt4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 79 kDa
7.5% SDS PAGE