Anti-Dishevelled 2 antibody (ab22616)
Key features and details
- Rabbit polyclonal to Dishevelled 2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dishevelled 2 antibody
See all Dishevelled 2 primary antibodies -
Description
Rabbit polyclonal to Dishevelled 2 -
Host species
Rabbit -
Specificity
ab22616 recognises Dishevelled 2. It does not recognises Dishevelled 1 or Dishevelled 3. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide:
(C)GAGRTGRPEERAPES
conjugated to KLH, corresponding to amino acids 600/614 of Human Dishevelled 2. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab22616 is a rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 79 kDa.
Note: dilute ab22616 to working concentration using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used). |
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 79 kDa. Note: dilute ab22616 to working concentration using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used). |
Target
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Function
Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. -
Sequence similarities
Belongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. -
Domain
The DIX domain mediates homooligomerization. -
Cellular localization
Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta. - Information by UniProt
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Database links
- Entrez Gene: 1856 Human
- Omim: 602151 Human
- SwissProt: O14641 Human
- Unigene: 118640 Human
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Alternative names
- Dishevelled 2 (homologous to Drosophila dsh) antibody
- Dishevelled dsh homolog 2 antibody
- dishevelled segment polarity protein 2 antibody
see all
Images
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Western blot - Anti-Dishevelled 2 antibody (ab22616)All lanes : Anti-Dishevelled 2 antibody (ab22616) at 1/1000 dilution
Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 79 kDa
Observed band size: 102 kDa why is the actual band size different from the predicted?
Datasheets and documents
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Datasheet download
References (9)
ab22616 has been referenced in 9 publications.
- Liao S et al. Aquaporin 9 inhibits growth and metastasis of hepatocellular carcinoma cells via Wnt/ß-catenin pathway. Aging (Albany NY) 12:1527-1544 (2020). PubMed: 31969493
- Du W et al. MiR-495 targeting dvl-2 represses the inflammatory response of ankylosing spondylitis. Am J Transl Res 11:2742-2753 (2019). PubMed: 31217850
- Zhang L et al. Dishevelled-2 modulates osteogenic differentiation of human synovial fibroblasts in osteoarthritis. Mol Med Rep 18:292-298 (2018). PubMed: 29749449
- Zhang K et al. Islr regulates canonical Wnt signaling-mediated skeletal muscle regeneration by stabilizing Dishevelled-2 and preventing autophagy. Nat Commun 9:5129 (2018). PubMed: 30510196
- Wang LH et al. The Antihelminthic Niclosamide Inhibits Cancer Stemness, Extracellular Matrix Remodeling, and Metastasis through Dysregulation of the Nuclear ß-catenin/c-Myc axis in OSCC. Sci Rep 8:12776 (2018). PubMed: 30143678
- Moreno-Fortuny A et al. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Biol Open 6:1592-1601 (2017). PubMed: 28923978
- Skalka N et al. Carboxypeptidase E: a negative regulator of the canonical Wnt signaling pathway. Oncogene : (2012). PubMed: 22824791
- Alten L et al. Differential regulation of node formation, nodal ciliogenesis and cilia positioning by Noto and Foxj1. Development 139:1276-84 (2012). PubMed: 22357932
- Mao Y et al. Preliminary identification of potential PDZ-domain proteins downstream of ephrin B2 during osteoclast differentiation of RAW264.7 cells. Int J Mol Med 27:669-77 (2011). PubMed: 21373749
