Anti-Dishevelled 2 antibody (ab22616)
Key features and details
- Rabbit polyclonal to Dishevelled 2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Dishevelled 2 antibody
See all Dishevelled 2 primary antibodies -
Description
Rabbit polyclonal to Dishevelled 2 -
Host species
Rabbit -
Specificity
ab22616 recognises Dishevelled 2. It does not recognises Dishevelled 1 or Dishevelled 3. -
Tested Applications & Species
See all applications and species dataApplication Species WB Human
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Immunogen
Synthetic peptide:
(C)GAGRTGRPEERAPES
conjugated to KLH, corresponding to amino acids 600/614 of Human Dishevelled 2. -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading... -
Purity
IgG fraction -
Purification notes
ab22616 is a rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab22616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| WB |
Human
|
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 79 kDa.
Note: dilute ab22616 to working concentration using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used). |
| Notes |
|---|
|
WB
1/1000. Predicted molecular weight: 79 kDa. Note: dilute ab22616 to working concentration using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used). |
Target
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Function
Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. -
Sequence similarities
Belongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. -
Domain
The DIX domain mediates homooligomerization. -
Cellular localization
Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta. - Information by UniProt
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Database links
- Entrez Gene: 1856 Human
- Omim: 602151 Human
- SwissProt: O14641 Human
- Unigene: 118640 Human
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Alternative names
- Dishevelled 2 (homologous to Drosophila dsh) antibody
- Dishevelled dsh homolog 2 antibody
- dishevelled segment polarity protein 2 antibody
see all
Images
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Western blot - Anti-Dishevelled 2 antibody (ab22616)All lanes : Anti-Dishevelled 2 antibody (ab22616) at 1/1000 dilution
Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 79 kDa
Observed band size: 102 kDa why is the actual band size different from the predicted?
Datasheets and documents
References (9)
ab22616 has been referenced in 9 publications.
- Liao S et al. Aquaporin 9 inhibits growth and metastasis of hepatocellular carcinoma cells via Wnt/ß-catenin pathway. Aging (Albany NY) 12:1527-1544 (2020). PubMed: 31969493
- Du W et al. MiR-495 targeting dvl-2 represses the inflammatory response of ankylosing spondylitis. Am J Transl Res 11:2742-2753 (2019). PubMed: 31217850
- Zhang L et al. Dishevelled-2 modulates osteogenic differentiation of human synovial fibroblasts in osteoarthritis. Mol Med Rep 18:292-298 (2018). PubMed: 29749449
- Zhang K et al. Islr regulates canonical Wnt signaling-mediated skeletal muscle regeneration by stabilizing Dishevelled-2 and preventing autophagy. Nat Commun 9:5129 (2018). PubMed: 30510196
- Wang LH et al. The Antihelminthic Niclosamide Inhibits Cancer Stemness, Extracellular Matrix Remodeling, and Metastasis through Dysregulation of the Nuclear ß-catenin/c-Myc axis in OSCC. Sci Rep 8:12776 (2018). PubMed: 30143678
- Moreno-Fortuny A et al. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Biol Open 6:1592-1601 (2017). PubMed: 28923978
- Skalka N et al. Carboxypeptidase E: a negative regulator of the canonical Wnt signaling pathway. Oncogene : (2012). PubMed: 22824791
- Alten L et al. Differential regulation of node formation, nodal ciliogenesis and cilia positioning by Noto and Foxj1. Development 139:1276-84 (2012). PubMed: 22357932
- Mao Y et al. Preliminary identification of potential PDZ-domain proteins downstream of ephrin B2 during osteoclast differentiation of RAW264.7 cells. Int J Mol Med 27:669-77 (2011). PubMed: 21373749
