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    dishevelled-2-antibody-ab22616.pdf

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Stem Cells Signaling Pathways Wnt Cytoplasmic
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Anti-Dishevelled 2 antibody (ab22616)

  • Datasheet
Reviews (1)Q&A (1)References (9)

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Western blot - Anti-Dishevelled 2 antibody (ab22616)

    Key features and details

    • Rabbit polyclonal to Dishevelled 2
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    You may also be interested in

    Protein
    Product image
    Recombinant Human Dishevelled 2 protein (ab152348)
    Secondary
    Product image
    Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    Knockout
    Human DVL2 knockout A549 cell line (ab277903)

    View more associated products

    Overview

    • Product name

      Anti-Dishevelled 2 antibody
      See all Dishevelled 2 primary antibodies
    • Description

      Rabbit polyclonal to Dishevelled 2
    • Host species

      Rabbit
    • Specificity

      ab22616 recognises Dishevelled 2. It does not recognises Dishevelled 1 or Dishevelled 3.
    • Tested Applications & Species

      Application Species
      WB
      Human
      See all applications and species data
    • Immunogen

      Synthetic peptide:

      (C)GAGRTGRPEERAPES

      conjugated to KLH, corresponding to amino acids 600/614 of Human Dishevelled 2.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
    • General notes

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
    • Storage buffer

      pH: 7.40
      Preservative: 0.065% Sodium azide
      Constituent: PBS
    • Concentration information loading...
    • Purity

      IgG fraction
    • Purification notes

      ab22616 is a rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation.
    • Clonality

      Polyclonal
    • Isotype

      IgG
    • Research areas

      • Stem Cells
      • Signaling Pathways
      • Wnt
      • Cytoplasmic
      • Stem Cells
      • Signaling Pathways
      • Notch
      • Cytoplasmic

    Associated products

    • Compatible Secondaries

      • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
      • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Isotype control

      • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
    • Recombinant Protein

      • Recombinant Human Dishevelled 2 protein (ab152348)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab22616 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    WB
    Human
    Application Abreviews Notes
    WB
    1/1000. Predicted molecular weight: 79 kDa.
    Note: dilute ab22616 to working concentration using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used).
    Notes
    WB
    1/1000. Predicted molecular weight: 79 kDa.
    Note: dilute ab22616 to working concentration using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used).

    Target

    • Function

      Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
    • Sequence similarities

      Belongs to the DSH family.
      Contains 1 DEP domain.
      Contains 1 DIX domain.
      Contains 1 PDZ (DHR) domain.
    • Domain

      The DIX domain mediates homooligomerization.
    • Cellular localization

      Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
    • Target information above from: UniProt accession O14641 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1856 Human
      • Omim: 602151 Human
      • SwissProt: O14641 Human
      • Unigene: 118640 Human
      • Alternative names

        • Dishevelled 2 (homologous to Drosophila dsh) antibody
        • Dishevelled dsh homolog 2 antibody
        • dishevelled segment polarity protein 2 antibody
        • Dishevelled-2 antibody
        • Dishevelled2 antibody
        • DSH homolog 2 antibody
        • DVL 2 antibody
        • Dvl2 antibody
        • DVL2_HUMAN antibody
        • Segment polarity protein dishevelled homolog DVL 2 antibody
        • Segment polarity protein dishevelled homolog DVL-2 antibody
        • Segment polarity protein dishevelled homolog DVL2 antibody
        see all

      Images

      • Western blot - Anti-Dishevelled 2 antibody (ab22616)
        Western blot - Anti-Dishevelled 2 antibody (ab22616)
        All lanes : Anti-Dishevelled 2 antibody (ab22616) at 1/1000 dilution

        Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
        Lane 2 : Human colon tissue lysate - total protein (ab30051)

        Lysates/proteins at 10 µg per lane.

        Secondary
        All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

        Predicted band size: 79 kDa
        Observed band size: 102 kDa
        why is the actual band size different from the predicted?

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (9)

      Publishing research using ab22616? Please let us know so that we can cite the reference in this datasheet.

      ab22616 has been referenced in 9 publications.

      • Liao S  et al. Aquaporin 9 inhibits growth and metastasis of hepatocellular carcinoma cells via Wnt/ß-catenin pathway. Aging (Albany NY) 12:1527-1544 (2020). PubMed: 31969493
      • Du W  et al. MiR-495 targeting dvl-2 represses the inflammatory response of ankylosing spondylitis. Am J Transl Res 11:2742-2753 (2019). PubMed: 31217850
      • Zhang L  et al. Dishevelled-2 modulates osteogenic differentiation of human synovial fibroblasts in osteoarthritis. Mol Med Rep 18:292-298 (2018). PubMed: 29749449
      • Zhang K  et al. Islr regulates canonical Wnt signaling-mediated skeletal muscle regeneration by stabilizing Dishevelled-2 and preventing autophagy. Nat Commun 9:5129 (2018). PubMed: 30510196
      • Wang LH  et al. The Antihelminthic Niclosamide Inhibits Cancer Stemness, Extracellular Matrix Remodeling, and Metastasis through Dysregulation of the Nuclear ß-catenin/c-Myc axis in OSCC. Sci Rep 8:12776 (2018). PubMed: 30143678
      • Moreno-Fortuny A  et al. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Biol Open 6:1592-1601 (2017). PubMed: 28923978
      • Skalka N  et al. Carboxypeptidase E: a negative regulator of the canonical Wnt signaling pathway. Oncogene : (2012). PubMed: 22824791
      • Alten L  et al. Differential regulation of node formation, nodal ciliogenesis and cilia positioning by Noto and Foxj1. Development 139:1276-84 (2012). PubMed: 22357932
      • Mao Y  et al. Preliminary identification of potential PDZ-domain proteins downstream of ephrin B2 during osteoclast differentiation of RAW264.7 cells. Int J Mol Med 27:669-77 (2011). PubMed: 21373749

      Customer reviews and Q&As

      Show All Reviews Q&A
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      1-2 of 2 Abreviews or Q&A

      IHC - Wholemount abreview for Anti-Dishevelled 2 antibody

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      IHC - Wholemount
      Sample
      Mouse Embryo (Mouse embryo neuroepithlium)
      Specification
      Mouse embryo neuroepithlium
      Read More

      Dr. Gabriel Galea

      Verified customer

      Submitted Jan 21 2020

      Question

      Konzentration von ab22616?

      Read More

      Abcam community

      Verified customer

      Asked on Jan 27 2012

      Answer

      Vielen Dank für Ihren Anruf.
      Mir wurde vom Labor bestätigt, dass die Konzentration dieses Antikörpers leider nicht ermittelbar ist, da er nur teilweise aufgereinigt ist. Eine Proteinmessung würde nicht die Konzentration des Antikörpers messen, sondern den totalen Proteingehalt.
      Als Anhaltspunkt in diesen Fällen empfehlen wir von einer Konzentration von 1mg/ml auszugehen und eine Verdünnung von ca. 1:500 und 1:1000 im ersten Experiment einzusetzen. Ausgehend von den Ergebnissen kann dann optimiert werden.
      Es tut mir leid, dass ich Ihnen dieses Mal keine präzisere Antwort geben konnte und wünsche viel Erfolg mit Ihrer Forschung.

      Read More

      Abcam Scientific Support

      Answered on Jan 27 2012

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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