Overview

  • Product name
    Anti-Dishevelled 2 antibody
    See all Dishevelled 2 primary antibodies
  • Description
    Rabbit polyclonal to Dishevelled 2
  • Host species
    Rabbit
  • Specificity
    ab22616 recognises Dishevelled 2. It does not recognises Dishevelled 1 or Dishevelled 3.
  • Tested applications
    Suitable for: ICC/IF, WBmore details
  • Species reactivity
    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide:

    (C)GAGRTGRPEERAPES

    conjugated to KLH, corresponding to amino acids 600/614 of Human Dishevelled 2.

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control
    • Dishevelled 2 transfected HEK293 cell line.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.065% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity
    IgG fraction
  • Purification notes
    ab22616 is a rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation.
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab22616 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB 1/1000. Predicted molecular weight: 79 kDa.
Note: dilute ab22616 to working concentration using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used).

Target

  • Function
    Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
  • Sequence similarities
    Belongs to the DSH family.
    Contains 1 DEP domain.
    Contains 1 DIX domain.
    Contains 1 PDZ (DHR) domain.
  • Domain
    The DIX domain mediates homooligomerization.
  • Cellular localization
    Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
  • Information by UniProt
  • Database links
    • Alternative names
      • Dishevelled 2 (homologous to Drosophila dsh) antibody
      • Dishevelled dsh homolog 2 antibody
      • dishevelled segment polarity protein 2 antibody
      • Dishevelled-2 antibody
      • Dishevelled2 antibody
      • DSH homolog 2 antibody
      • DVL 2 antibody
      • Dvl2 antibody
      • DVL2_HUMAN antibody
      • Segment polarity protein dishevelled homolog DVL 2 antibody
      • Segment polarity protein dishevelled homolog DVL-2 antibody
      • Segment polarity protein dishevelled homolog DVL2 antibody
      see all

    Images

    • Western blot - Anti-Dishevelled 2 antibody (ab22616)
      Western blot - Anti-Dishevelled 2 antibody (ab22616)
      All lanes : Anti-Dishevelled 2 antibody (ab22616) at 1/1000 dilution

      Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
      Lane 2 : Human colon tissue lysate - total protein (ab30051)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Predicted band size: 79 kDa
      Observed band size: 102 kDa (why is the actual band size different from the predicted?)

    • Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 2 antibody (ab22616)
      Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 2 antibody (ab22616)
      ICC/IF image of ab22616 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22616, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

    References

    This product has been referenced in:
    • Moreno-Fortuny A  et al. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Biol Open 6:1592-1601 (2017). Read more (PubMed: 28923978) »
    • Skalka N  et al. Carboxypeptidase E: a negative regulator of the canonical Wnt signaling pathway. Oncogene : (2012). Read more (PubMed: 22824791) »

    See all 3 Publications for this product

    Publishing research using ab22616? Please let us know so that we can cite the reference in this datasheet.

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    Konzentration von ab22616?

    Vielen Dank für Ihren Anruf.
    Mir wurde vom Labor bestätigt, dass die Konzentration dieses Antikörpers leider nicht ermittelbar ist, da er nur teilweise aufgereinigt ist. Eine Proteinmessung würde nicht die Konzentration des Antikörpers messen, son...

    Read More

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