Anti-Dishevelled 2 antibody (ab22616)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Dishevelled 2 antibody
See all Dishevelled 2 primary antibodies -
DescriptionRabbit polyclonal to Dishevelled 2
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Host speciesRabbit
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Specificityab22616 recognises Dishevelled 2. It does not recognises Dishevelled 1 or Dishevelled 3.
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Tested applicationsSuitable for: ICC/IF, WBmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide:
(C)GAGRTGRPEERAPES
conjugated to KLH, corresponding to amino acids 600/614 of Human Dishevelled 2. -
Positive control
- Dishevelled 2 transfected HEK293 cell line.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityIgG fraction
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Purification notesab22616 is a rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab22616 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | Use at an assay dependent concentration. | |
| WB | 1/1000. Predicted molecular weight: 79 kDa. Note: dilute ab22616 to working concentration using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used). |
Target
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FunctionParticipates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
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Sequence similaritiesBelongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. -
DomainThe DIX domain mediates homooligomerization.
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Cellular localizationCell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
- Information by UniProt
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Database links
- Entrez Gene: 1856 Human
- Entrez Gene: 13543 Mouse
- Omim: 602151 Human
- SwissProt: O14641 Human
- SwissProt: Q60838 Mouse
- Unigene: 118640 Human
- Unigene: 5114 Mouse
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Alternative names
- Dishevelled 2 (homologous to Drosophila dsh) antibody
- Dishevelled dsh homolog 2 antibody
- dishevelled segment polarity protein 2 antibody
see all
Images
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Western blot - Anti-Dishevelled 2 antibody (ab22616)All lanes : Anti-Dishevelled 2 antibody (ab22616) at 1/1000 dilution
Lane 1 : THP1 (Human acute monocytic leukemia cell line) Whole Cell Lysate
Lane 2 : Human colon tissue lysate - total protein (ab30051)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Predicted band size: 79 kDa
Observed band size: 102 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Dishevelled 2 antibody (ab22616)ICC/IF image of ab22616 stained SKNSH cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab22616, 1/200 dilution) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Datasheets and documents
References
This product has been referenced in:
- Zhang L et al. Dishevelled-2 modulates osteogenic differentiation of human synovial fibroblasts in osteoarthritis. Mol Med Rep 18:292-298 (2018). Read more (PubMed: 29749449) »
- Moreno-Fortuny A et al. MCAM contributes to the establishment of cell autonomous polarity in myogenic and chondrogenic differentiation. Biol Open 6:1592-1601 (2017). Read more (PubMed: 28923978) »
