- Datasheet
- Specific References
- Protocols
Overview
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Product nameAnti-Dishevelled 2 antibody
See all Dishevelled 2 primary antibodies -
DescriptionRabbit polyclonal to Dishevelled 2
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Host speciesRabbit
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Specificityab22618 recognises dishevelled 2. It doesn't cross-react with dishevelled 1 or dishevelled 3.
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Tested applicationsSuitable for: WBmore details
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Species reactivityReacts with: Mouse, Human
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Immunogen
Synthetic peptide:
RGGEASGTSDGGP(C)
conjugated to KLH by a MBS linker, corresponding to amino acids 633/645 of Human Dishevelled 2. -
Positive control
- Dishevelled 2 transfected HEK293 cell lysate.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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Storage bufferpH: 7.40
Preservative: 0.065% Sodium azide
Constituent: PBS -
Concentration information loading... -
PurityIgG fraction
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Purification notesab22618 is a rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation.
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ClonalityPolyclonal
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IsotypeIgG
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Research areas
Associated products
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Compatible Secondaries
Applications
Our Abpromise guarantee covers the use of ab22618 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Application notes
WB: 1/1000. Predicted molecular weight: 18 kDa.
Note: dilute ab22618 to working strength using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Note: dilute ab22618 to working strength using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used).
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
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FunctionParticipates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
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Sequence similaritiesBelongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. -
DomainThe DIX domain mediates homooligomerization.
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Cellular localizationCell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
- Information by UniProt
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Database links
- Entrez Gene: 1856 Human
- Entrez Gene: 13543 Mouse
- Omim: 602151 Human
- SwissProt: O14641 Human
- SwissProt: Q60838 Mouse
- Unigene: 118640 Human
- Unigene: 5114 Mouse
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Alternative names
- Dishevelled 2 (homologous to Drosophila dsh) antibody
- Dishevelled dsh homolog 2 antibody
- dishevelled segment polarity protein 2 antibody
see all
Images
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Immunohistochemistry (Frozen sections) - Anti-Dishevelled 2 antibody (ab22618)Image courtesy of an anonymous Abreview.ab22618 staining Dishevelled 2 in murine rib cartilage by Immunohistochemistry (Frozen sections). Tissue was fixed with paraformaldehyde and permeabilized using 0.1% Triton. Samples were then blocked with 20% serum for 1 hour at 19°C followed by incubation with the primary antibody at a 1/100 dilution for 16 hours at 4°C. An Alexa Fluor® conjugated donkey anti-rabbit polyclonal was used as secondary antibody at a 1/200 dilution. Counterstain DAPI (blue).
Protocols
References
ab22618 has not yet been referenced specifically in any publications.
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"