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    dishevelled-2-antibody-ab22618.pdf

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Stem Cells Signaling Pathways Wnt Cytoplasmic
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Anti-Dishevelled 2 antibody (ab22618)

  • Datasheet
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Key features and details

  • Rabbit polyclonal to Dishevelled 2
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human Dishevelled 2 protein (ab152348)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Dishevelled 2 antibody
    See all Dishevelled 2 primary antibodies
  • Description

    Rabbit polyclonal to Dishevelled 2
  • Host species

    Rabbit
  • Specificity

    ab22618 recognises dishevelled 2. It doesn't cross-react with dishevelled 1 or dishevelled 3.
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide:

    RGGEASGTSDGGP(C)

    conjugated to KLH by a MBS linker, corresponding to amino acids 633/645 of Human Dishevelled 2.
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • Dishevelled 2 transfected HEK293 cell lysate.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer

    pH: 7.40
    Preservative: 0.065% Sodium azide
    Constituent: PBS
  • Concentration information loading...
  • Purity

    IgG fraction
  • Purification notes

    ab22618 is a rabbit antiserum partially purified by caprylic acid and ammonium sulphate precipitation.
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Cytoplasmic
    • Stem Cells
    • Signaling Pathways
    • Notch
    • Cytoplasmic

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human Dishevelled 2 protein (ab152348)

Applications

  • Application notes
    WB: 1/1000. Predicted molecular weight: 18 kDa.

    Note: dilute ab22618 to working strength using 50mM PBS (pH 7.4) containing 1.5% sodium chloride and 1% normal goat serum (if a goat antirabbit IgG linker antibody is to be used).

    Not tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
    • Sequence similarities

      Belongs to the DSH family.
      Contains 1 DEP domain.
      Contains 1 DIX domain.
      Contains 1 PDZ (DHR) domain.
    • Domain

      The DIX domain mediates homooligomerization.
    • Cellular localization

      Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
    • Target information above from: UniProt accession O14641 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 1856 Human
      • Omim: 602151 Human
      • SwissProt: O14641 Human
      • Unigene: 118640 Human
      • Alternative names

        • Dishevelled 2 (homologous to Drosophila dsh) antibody
        • Dishevelled dsh homolog 2 antibody
        • dishevelled segment polarity protein 2 antibody
        • Dishevelled-2 antibody
        • Dishevelled2 antibody
        • DSH homolog 2 antibody
        • DVL 2 antibody
        • Dvl2 antibody
        • DVL2_HUMAN antibody
        • Segment polarity protein dishevelled homolog DVL 2 antibody
        • Segment polarity protein dishevelled homolog DVL-2 antibody
        • Segment polarity protein dishevelled homolog DVL2 antibody
        see all

      Protocols

      • Western blot protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
    • References (0)

      Publishing research using ab22618? Please let us know so that we can cite the reference in this datasheet.

      ab22618 has not yet been referenced specifically in any publications.

      Customer reviews and Q&As

      Show All Reviews Q&A
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      Immunohistochemistry (Frozen sections) abreview for Anti-Dishevelled 2 antibody

      Average
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Frozen sections)
      Sample
      Mouse Tissue sections (Rib cartilage)
      Specification
      Rib cartilage
      Fixative
      Paraformaldehyde
      Permeabilization
      Yes - 0.1% Triton
      Blocking step
      Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 20% · Temperature: 19°C
      Read More

      Abcam user community

      Verified customer

      Submitted Jan 25 2011

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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