Overview

  • Product name

    Anti-Dishevelled 2 antibody
    See all Dishevelled 2 primary antibodies
  • Description

    Rabbit polyclonal to Dishevelled 2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Cow, Rhesus monkey
  • Immunogen

    Recombinant fragment within Human Dishevelled 2 (internal sequence). The exact sequence is proprietary.
    Database link: O14641

  • Positive control

    • ICC/IF: HCT 116 cells. WB: Neuro-2a, C8D30, NIH/3T3, RAW 264.7, C2C12, PC-12, Rat2, Jurkat, Raji, NCI-H929, HEK-293T, A431, HeLa and HepG2 whole cell extracts.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab228804 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/20000. Predicted molecular weight: 78 kDa.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
  • Sequence similarities

    Belongs to the DSH family.
    Contains 1 DEP domain.
    Contains 1 DIX domain.
    Contains 1 PDZ (DHR) domain.
  • Domain

    The DIX domain mediates homooligomerization.
  • Cellular localization

    Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dishevelled 2 (homologous to Drosophila dsh) antibody
    • Dishevelled dsh homolog 2 antibody
    • dishevelled segment polarity protein 2 antibody
    • Dishevelled-2 antibody
    • Dishevelled2 antibody
    • DSH homolog 2 antibody
    • DVL 2 antibody
    • Dvl2 antibody
    • DVL2_HUMAN antibody
    • Segment polarity protein dishevelled homolog DVL 2 antibody
    • Segment polarity protein dishevelled homolog DVL-2 antibody
    • Segment polarity protein dishevelled homolog DVL2 antibody
    see all

Images

  • HCT 116 (human colorectal carcinoma cell line) cells stained for Dishevelled 2 (green) using ab228804 at 1/500 dilution in ICC/IF. Cells were fixed in 4% paraformaldehyde at RT for 15 minutes.

    Blue: Hoechst 33342 staining.

  • All lanes : Anti-Dishevelled 2 antibody (ab228804) at 1/5000 dilution

    Lane 1 : Neuro-2a (mouse neuroblastoma cell line) whole cell extract
    Lane 2 : C8D30 whole cell extract
    Lane 3 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell extract
    Lane 4 : RAW 264.7 (mouse macrophage cell line transformed with Abelson murine leukemia virus) whole cell extract
    Lane 5 : C2C12 (mouse myoblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 78 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-Dishevelled 2 antibody (ab228804) at 1/5000 dilution

    Lane 1 : PC-12 (rat adrenal gland pheochromocytoma cell line) whole cell extract
    Lane 2 : Rat2 (rat fibroblast cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 78 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-Dishevelled 2 antibody (ab228804) at 1/10000 dilution

    Lane 1 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell extract
    Lane 2 : Raji (human Burkitt's lymphoma cell line) whole cell extract
    Lane 3 : NCI-H929 whole cell extract

    Lysates/proteins at 30 µg per lane.

    Secondary
    All lanes : HRP-conjugated anti-rabbit IgG

    Predicted band size: 78 kDa



    7.5% SDS-PAGE gel.

  • All lanes : Anti-Dishevelled 2 antibody (ab228804) at 1/5000 dilution

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell extract
    Lane 2 : A431 (human epidermoid carcinoma cell line) whole cell extract
    Lane 3 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell extract
    Lane 4 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell extract

    Lysates/proteins at 30 µg per lane.

    Predicted band size: 78 kDa



    7.5% SDS-PAGE gel.

References

ab228804 has not yet been referenced specifically in any publications.

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