Recombinant
RabMAb

Recombinant Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933)

Overview

  • Product name

    Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145]
    See all Dishevelled 2 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR145] to Dishevelled 2 (phospho S143)
  • Host species

    Rabbit
  • Specificity

    ab124933 only detects Dishevelled 2 phosphorylated at Serine 143.
  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human Dishevelled 2 (phospho S143).
    (Peptide available as ab173138)

  • Positive control

    • WB: F9 and HEK293 cell lysates.
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 40% Glycerol, 0.05% BSA, 59% PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR145
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124933 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).Can be blocked with Dishevelled 2 peptide (ab173138).

For unpurified use at 1/1000 - 1/10000.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.
  • Target

    • Function

      Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
    • Sequence similarities

      Belongs to the DSH family.
      Contains 1 DEP domain.
      Contains 1 DIX domain.
      Contains 1 PDZ (DHR) domain.
    • Domain

      The DIX domain mediates homooligomerization.
    • Cellular localization

      Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
    • Information by UniProt
    • Database links

    • Alternative names

      • Dishevelled 2 (homologous to Drosophila dsh) antibody
      • Dishevelled dsh homolog 2 antibody
      • dishevelled segment polarity protein 2 antibody
      • Dishevelled-2 antibody
      • Dishevelled2 antibody
      • DSH homolog 2 antibody
      • DVL 2 antibody
      • Dvl2 antibody
      • DVL2_HUMAN antibody
      • Segment polarity protein dishevelled homolog DVL 2 antibody
      • Segment polarity protein dishevelled homolog DVL-2 antibody
      • Segment polarity protein dishevelled homolog DVL2 antibody
      see all

    Images

    • All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (purified)

      Lane 1 : Untreated F9 cell lysate
      Lane 2 : Alkaline phosphatase treated F9 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 78 kDa
      Observed band size: 95 kDa
      why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/5000 dilution (purified)

      Lane 1 : Untreated HEK293 cell lysate
      Lane 2 : Alkaline phosphatase treated HEK293 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution

      Predicted band size: 78 kDa
      Observed band size: 95 kDa why is the actual band size different from the predicted?



      Blocking and dilution buffer: 5% NFDM/TBST.

    • All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (unpurified)

      Lane 1 : F9 cell lysate untreated
      Lane 2 : F9 cell lysate treated with Alkaline Phosphatase

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution

      Predicted band size: 78 kDa
      Observed band size: 95 kDa why is the actual band size different from the predicted?

    References

    This product has been referenced in:

    See all 4 Publications for this product

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