Recombinant
RabMAb

Recombinant Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)

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Overview

  • Product name

    Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free
    See all Dishevelled 2 primary antibodies

  • Description

    Rabbit monoclonal [EPNCIR145] to Dishevelled 2 (phospho S143) - BSA and Azide free

  • Host species

    Rabbit

  • Specificity

    This antibody only detects Dishevelled 2 phosphorylated at Serine 143.

  • Tested applications

    Suitable for: WBmore details
    Unsuitable for: Flow Cyt,ICC/IF or IHC-P

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide within Human Dishevelled 2 (phospho S143). The exact sequence is proprietary.
    (Peptide available as ab173138)

  • General notes

     

    ab169950 is the carrier-free version of ab124933 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab169950 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.

    Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab169950 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).Can be blocked with Dishevelled 2 peptide (ab173138).
  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IHC-P.

    • Target

    • Function

      Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.

    • Sequence similarities

      Belongs to the DSH family.
      Contains 1 DEP domain.
      Contains 1 DIX domain.
      Contains 1 PDZ (DHR) domain.

    • Domain

      The DIX domain mediates homooligomerization.

    • Cellular localization

      Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
    • Information by UniProt

    • Database links

      • Alternative names

        • Dishevelled 2 (homologous to Drosophila dsh) antibody
        • Dishevelled dsh homolog 2 antibody
        • dishevelled segment polarity protein 2 antibody
        • Dishevelled-2 antibody
        • Dishevelled2 antibody
        • DSH homolog 2 antibody
        • DVL 2 antibody
        • Dvl2 antibody
        • DVL2_HUMAN antibody
        • Segment polarity protein dishevelled homolog DVL 2 antibody
        • Segment polarity protein dishevelled homolog DVL-2 antibody
        • Segment polarity protein dishevelled homolog DVL2 antibody
        see all

      References

      ab169950 has not yet been referenced specifically in any publications.

      Publishing research using ab169950? Please let us know so that we can cite the reference in this datasheet.

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