Recombinant Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPNCIR145] to Dishevelled 2 (phospho S143) - BSA and Azide free
- Suitable for: WB
- Reacts with: Mouse, Human
Overview
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Product name
Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free
See all Dishevelled 2 primary antibodies -
Description
Rabbit monoclonal [EPNCIR145] to Dishevelled 2 (phospho S143) - BSA and Azide free -
Host species
Rabbit -
Specificity
This antibody only detects Dishevelled 2 phosphorylated at Serine 143.
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Tested applications
Suitable for: WBmore details
Unsuitable for: Flow Cyt,ICC/IF or IHC-P -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide within Human Dishevelled 2 (phospho S143). The exact sequence is proprietary.
(Peptide available asab173138) -
General notes
ab169950 is the carrier-free version of ab124933 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
Ab169950 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.
Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPNCIR145 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Conjugation kits
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Immunizing Peptide (Blocking)
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab169950 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | Use at an assay dependent concentration. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).Can be blocked with Dishevelled 2 peptide (ab173138). |
Target
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Function
Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes. -
Sequence similarities
Belongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain. -
Domain
The DIX domain mediates homooligomerization. -
Cellular localization
Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta. - Information by UniProt
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Database links
- Entrez Gene: 1856 Human
- Entrez Gene: 13543 Mouse
- Omim: 602151 Human
- SwissProt: O14641 Human
- SwissProt: Q60838 Mouse
- Unigene: 118640 Human
- Unigene: 5114 Mouse
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Alternative names
- Dishevelled 2 (homologous to Drosophila dsh) antibody
- Dishevelled dsh homolog 2 antibody
- dishevelled segment polarity protein 2 antibody
see all
Images
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Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (purified)
Lane 1 : Untreated F9 cell lysate
Lane 2 : Alkaline phosphatase treated F9 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?This data was developed using ab124933, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/5000 dilution (purified)
Lane 1 : Untreated HEK293 cell lysate
Lane 2 : Alkaline phosphatase treated HEK293 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Peroxidase-conjugated goat anti-rabbit IgG, (H+L) at 1/1000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?This data was developed using ab124933, the same antibody clone in a different buffer formulation.
Blocking and dilution buffer: 5% NFDM/TBST.
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Western blot - Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] - BSA and Azide free (ab169950)All lanes : Anti-Dishevelled 2 (phospho S143) antibody [EPNCIR145] (ab124933) at 1/1000 dilution (unpurified)
Lane 1 : F9 cell lysate untreated
Lane 2 : F9 cell lysate treated with Alkaline Phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP-conjugated goat anti-rabbit IgG at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?This data was developed using ab124933, the same antibody clone in a different buffer formulation.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
References (0)
ab169950 has not yet been referenced specifically in any publications.