Product nameAnti-Dishevelled 2 (phospho T224) antibody [EPNCIR146]
See all Dishevelled 2 primary antibodies
DescriptionRabbit monoclonal [EPNCIR146] to Dishevelled 2 (phospho T224)
Specificityab124941 only detects Dishevelled 2 phosphorylated at Threonine 224.
Tested applicationsSuitable for: WB, IPmore details
Unsuitable for: Flow Cyt,ICC or IHC-P
Species reactivityReacts with: Mouse, Rat, Human
Synthetic phosphopeptide corresponding to residues surrounding Threonine 224 of Human Dishevelled 2.
- HeLa cell lysate treated with Calyculin A
This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab124941 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).|
|IP||1/10 - 1/100.|
FunctionParticipates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
Sequence similaritiesBelongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain.
DomainThe DIX domain mediates homooligomerization.
Cellular localizationCell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
- Information by UniProt
- Dishevelled 2 (homologous to Drosophila dsh) antibody
- Dishevelled dsh homolog 2 antibody
- dishevelled segment polarity protein 2 antibody
All lanes : Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941) at 1/1000 dilution
Lane 1 : HeLa cell lysate untreated
Lane 2 : HeLa cell lysate treated with Calyculin A
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution
Predicted band size: 78 kDa
Observed band size: 95 kDa why is the actual band size different from the predicted?
ab124941 has not yet been referenced specifically in any publications.