Recombinant
RabMAb

Recombinant Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941)

Overview

  • Product name

    Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146]
    See all Dishevelled 2 primary antibodies
  • Description

    Rabbit monoclonal [EPNCIR146] to Dishevelled 2 (phospho T224)
  • Host species

    Rabbit
  • Specificity

    ab124941 only detects Dishevelled 2 phosphorylated at Threonine 224.
  • Tested applications

    Suitable for: WB, IPmore details
    Unsuitable for: Flow Cyt,ICC or IHC-P
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic phosphopeptide corresponding to residues surrounding Threonine 224 of Human Dishevelled 2.

  • Positive control

    • HeLa cell lysate treated with Calyculin A
  • General notes

    This antibody was developed as part of a collaboration between Epitomics, the National Cancer Institute's Center for Cancer Research and the lab of Kyung Lee. View antibodies from NCI Center for Cancer Research Collaboration.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPNCIR146
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab124941 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 95 kDa (predicted molecular weight: 78 kDa).
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt,ICC or IHC-P.
  • Target

    • Function

      Participates in Wnt signaling by binding to the cytoplasmic C-terminus of frizzled family members and transducing the Wnt signal to down-stream effectors. Promotes internalization and degradation of frizzled proteins upon Wnt signaling. Plays a role both in canonical and non-canonical Wnt signaling. Plays a role in the signal transduction pathways mediated by multiple Wnt genes.
    • Sequence similarities

      Belongs to the DSH family.
      Contains 1 DEP domain.
      Contains 1 DIX domain.
      Contains 1 PDZ (DHR) domain.
    • Domain

      The DIX domain mediates homooligomerization.
    • Cellular localization

      Cell membrane. Cytoplasm > cytosol. Cytoplasmic vesicle. Localizes at the cell membrane upon interaction with frizzled family members and promotes their internalization. Localizes to cytoplasmic puncta.
    • Information by UniProt
    • Database links

    • Alternative names

      • Dishevelled 2 (homologous to Drosophila dsh) antibody
      • Dishevelled dsh homolog 2 antibody
      • dishevelled segment polarity protein 2 antibody
      • Dishevelled-2 antibody
      • Dishevelled2 antibody
      • DSH homolog 2 antibody
      • DVL 2 antibody
      • Dvl2 antibody
      • DVL2_HUMAN antibody
      • Segment polarity protein dishevelled homolog DVL 2 antibody
      • Segment polarity protein dishevelled homolog DVL-2 antibody
      • Segment polarity protein dishevelled homolog DVL2 antibody
      see all

    Images

    • All lanes : Anti-Dishevelled 2 (phospho T224) antibody [EPNCIR146] (ab124941) at 1/1000 dilution

      Lane 1 : HeLa cell lysate untreated
      Lane 2 : HeLa cell lysate treated with Calyculin A

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled Goat anti Rabbit IgG at 1/2000 dilution

      Predicted band size: 78 kDa
      Observed band size: 95 kDa
      why is the actual band size different from the predicted?

    References

    ab124941 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab124941.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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