Key features and details
- Rabbit polyclonal to Dishevelled 3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dishevelled 3 antibody
See all Dishevelled 3 primary antibodies
DescriptionRabbit polyclonal to Dishevelled 3
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide corresponding to Human Dishevelled 3 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available as
- Recombinant Human Dishevelled 3 protein (ab114497) can be used as a positive control in WB. This antibody gave a positive signal in the following lysates: Ovary (Human) Tissue Lysate; Heart (Human) Tissue Lysate
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab93850 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 mg/ml. Detects a band of approximately 78 kDa (predicted molecular weight: 78 kDa).|
FunctionMay play a role in the signal transduction pathway mediated by multiple Wnt genes.
Sequence similaritiesBelongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain.
modificationsUbiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
- Information by UniProt
- Dishevelled 3 (homologous to Drosophila dsh) antibody
- Dishevelled 3 antibody
- Dishevelled 3, dsh homolog (Drosophila) antibody
All lanes : Anti-Dishevelled 3 antibody (ab93850) at 1 µg/ml
Lane 1 : Human ovary tissue lysate - total protein (ab30222)
Lane 2 : Human heart tissue lysate - total protein (ab29431)
Lysates/proteins at 10 µg per lane.
All lanes : Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 78 kDa
Observed band size: 78 kDa
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
ab93850 has not yet been referenced specifically in any publications.