Key features and details
- Rabbit polyclonal to Dishevelled 3 - N-terminal
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Dishevelled 3 antibody - N-terminal
See all Dishevelled 3 primary antibodies
DescriptionRabbit polyclonal to Dishevelled 3 - N-terminal
Tested applicationsSuitable for: WB, IHC-P, ICC/IFmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Recombinant fragment corresponding to a region within N-terminal amino acids 1-166 of Human Dishevelled 3 (Uniprot ID: Q92997).
- Human SW480 xenograft tissue; HCT116 cells; HCT116 whole cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab152161 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/5000 - 1/20000. Predicted molecular weight: 78 kDa.|
|IHC-P||1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval also possible using Tris-EDTA buffer (pH8.0).|
|ICC/IF||1/100 - 1/1000.|
FunctionMay play a role in the signal transduction pathway mediated by multiple Wnt genes.
Sequence similaritiesBelongs to the DSH family.
Contains 1 DEP domain.
Contains 1 DIX domain.
Contains 1 PDZ (DHR) domain.
modificationsUbiquitinated. Deubiquitinated by CYLD, which acts on 'Lys-63'-linked ubiquitin chains.
- Information by UniProt
- Dishevelled 3 (homologous to Drosophila dsh) antibody
- Dishevelled 3 antibody
- Dishevelled 3, dsh homolog (Drosophila) antibody
Anti-Dishevelled 3 antibody - N-terminal (ab152161) at 1/10000 dilution + HCT116 whole cell lysate at 30 µg
Predicted band size: 78 kDa
7.5% SDS PAGE
Immunohistochemical analysis of paraffin-embedded Human SW480 xenograft tissue, staining Dishevelled 3 using ab152161 at a 1/100 dilution.
Immunofluorescence analysis of methanol-fixed HCT116 cells, staining Dishevelled 3 (green) using ab152161 at a 1/500 dilution.
ab152161 has not yet been referenced specifically in any publications.