Anti-DIXDC1 antibody (ab226210)
Key features and details
- Rabbit polyclonal to DIXDC1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DIXDC1 antibody -
Description
Rabbit polyclonal to DIXDC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey -
Immunogen
Synthetic peptide within Human DIXDC1 aa 633-683. The exact sequence is proprietary. NP_001033043.1
Database link: Q155Q3-1 -
Positive control
- IP: HEK-293T whole cell lysate. WB: HeLa and HEK-293T whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8 -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab226210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000 - 1/5000. Predicted molecular weight: 77 kDa.
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/1000 - 1/5000. Predicted molecular weight: 77 kDa. |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Positive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2. -
Tissue specificity
Ubiquitously expressed with higher expression in cardiac and skeletal muscles. -
Sequence similarities
Belongs to the DIXDC1 family.
Contains 1 CH (calponin-homology) domain.
Contains 1 DIX domain. -
Domain
The coiled-coil domain mediates interaction with MAP3K4 and inhibition of AXIN1-mediated JNK activation through MAP3K4.
The DIX domain mediates interaction with AXIN1 and inhibition of AXIN1-mediated JNK activation through MAP3K1. Mediates interaction with DVL2; this interaction is required for activation of Wnt signaling. -
Post-translational
modificationsPhosphorylated on tyrosine and serine residues.
Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation. -
Cellular localization
Cytoplasm; Cell junction > focal adhesion. Associated with actin stress fiber at the filament ends and Cytoplasm > cytosol. Colocalizes with gamma-tubulin at the centrosome, both during interphase and mitosis. - Information by UniProt
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Database links
- Entrez Gene: 419798 Chicken
- Entrez Gene: 466779 Chimpanzee
- Entrez Gene: 541211 Cow
- Entrez Gene: 608957 Dog
- Entrez Gene: 85458 Human
- Entrez Gene: 710847 Rhesus monkey
- Omim: 610493 Human
- SwissProt: Q155Q3 Human
see all -
Alternative names
- CCD1 antibody
- Ccd1Aalpha1L antibody
- Ccd1Abeta1L antibody
see all
Images
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All lanes : Anti-DIXDC1 antibody (ab226210) at 0.4 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 3 minutes -
DIXDC1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226210 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226210 at 1 µg/ml.
Lane 1: ab226210 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab226210 has been referenced in 1 publication.
- Huang CQ et al. Circular RNA circ_0000423 promotes gastric cancer cell proliferation, migration and invasion via the microR-582-3p/Disheveled-Axin domain containing 1 axis. Bioengineered 12:12755-12766 (2021). PubMed: 34898351