Key features and details
- Rabbit polyclonal to DIXDC1
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DIXDC1 antibody
See all DIXDC1 primary antibodies
DescriptionRabbit polyclonal to DIXDC1
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey
Synthetic peptide within Human DIXDC1 aa 633-683. The exact sequence is proprietary. NP_001033043.1
Database link: Q155Q3-1
- IP: HEK-293T whole cell lysate. WB: HeLa and HEK-293T whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab226210 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Predicted molecular weight: 77 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionPositive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2.
Tissue specificityUbiquitously expressed with higher expression in cardiac and skeletal muscles.
Sequence similaritiesBelongs to the DIXDC1 family.
Contains 1 CH (calponin-homology) domain.
Contains 1 DIX domain.
DomainThe coiled-coil domain mediates interaction with MAP3K4 and inhibition of AXIN1-mediated JNK activation through MAP3K4.
The DIX domain mediates interaction with AXIN1 and inhibition of AXIN1-mediated JNK activation through MAP3K1. Mediates interaction with DVL2; this interaction is required for activation of Wnt signaling.
modificationsPhosphorylated on tyrosine and serine residues.
Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation.
Cellular localizationCytoplasm; Cell junction > focal adhesion. Associated with actin stress fiber at the filament ends and Cytoplasm > cytosol. Colocalizes with gamma-tubulin at the centrosome, both during interphase and mitosis.
- Information by UniProt
- CCD1 antibody
- Ccd1Aalpha1L antibody
- Ccd1Abeta1L antibody
All lanes : Anti-DIXDC1 antibody (ab226210) at 0.4 µg/ml
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 77 kDa
Exposure time: 3 minutes
DIXDC1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226210 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226210 at 1 µg/ml.
Lane 1: ab226210 IP in HEK-293T whole cell lysate.
Lane 2: Control IgG IP in HEK-293T whole cell lysate.
Detection: Chemiluminescence with exposure time of 3 minutes.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab226210 has not yet been referenced specifically in any publications.