Overview

  • Product name

  • Description

    Rabbit polyclonal to DIXDC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Pig, Chimpanzee, Zebrafish, Rhesus monkey
  • Immunogen

    Synthetic peptide within Human DIXDC1 aa 633-683. The exact sequence is proprietary. NP_001033043.1
    Database link: Q155Q3-1

  • Positive control

    • IP: HEK-293T whole cell lysate. WB: HeLa and HEK-293T whole cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab226210 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Predicted molecular weight: 77 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Positive effector of the Wnt signaling pathway; activates WNT3A signaling via DVL2. Regulates JNK activation by AXIN1 and DVL2.
  • Tissue specificity

    Ubiquitously expressed with higher expression in cardiac and skeletal muscles.
  • Sequence similarities

    Belongs to the DIXDC1 family.
    Contains 1 CH (calponin-homology) domain.
    Contains 1 DIX domain.
  • Domain

    The coiled-coil domain mediates interaction with MAP3K4 and inhibition of AXIN1-mediated JNK activation through MAP3K4.
    The DIX domain mediates interaction with AXIN1 and inhibition of AXIN1-mediated JNK activation through MAP3K1. Mediates interaction with DVL2; this interaction is required for activation of Wnt signaling.
  • Post-translational
    modifications

    Phosphorylated on tyrosine and serine residues.
    Polyubiquitinated, leading to its proteasomal degradation. WNT3A signaling increases DIXDC1 protein levels by inhibiting its ubiquitination and subsequent degradation.
  • Cellular localization

    Cytoplasm; Cell junction > focal adhesion. Associated with actin stress fiber at the filament ends and Cytoplasm > cytosol. Colocalizes with gamma-tubulin at the centrosome, both during interphase and mitosis.
  • Information by UniProt
  • Database links

  • Alternative names

    • CCD1 antibody
    • Ccd1Aalpha1L antibody
    • Ccd1Abeta1L antibody
    • Ccd1BalphaL antibody
    • Ccd1BbetaL antibody
    • Ccd1CL antibody
    • Coiled coil DIX1 antibody
    • Coiled coil protein DIX1 antibody
    • Coiled-coil protein DIX1 antibody
    • Coiled-coil-DIX1 antibody
    • DIX domain containing 1 antibody
    • DIX domain containing protein 1 antibody
    • DIX domain-containing protein 1 antibody
    • DIXC1_HUMAN antibody
    • DIXDC1 antibody
    • Dixin antibody
    • KIAA1735 antibody
    see all

Images

  • All lanes : Anti-DIXDC1 antibody (ab226210) at 0.4 µg/ml

    Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
    Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
    Lane 3 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 77 kDa


    Exposure time: 3 minutes
  • DIXDC1 was immunoprecipitated from HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate (1 mg for IP, 20% of IP loaded) with ab226210 at 6 µg/mg lysate. Western blot was performed from the immunoprecipitate using ab226210 at 1 µg/ml.

    Lane 1: ab226210 IP in HEK-293T whole cell lysate.

    Lane 2: Control IgG IP in HEK-293T whole cell lysate.

    Detection:  Chemiluminescence with exposure time of 3 minutes.

References

ab226210 has not yet been referenced specifically in any publications.

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