Key features and details
- Rabbit polyclonal to DjC8
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DjC8 antibody
See all DjC8 primary antibodies
DescriptionRabbit polyclonal to DjC8
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Dog, Turkey, Pig, Chimpanzee, Rhesus monkey, Orangutan, Platypus
Synthetic peptide corresponding to a region between residue 203 and 253 of Human DjC8 (NP_055095.2).
- Whole cell lysate from HeLa cells and 293T cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab86138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 30 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
Sequence similaritiesContains 1 J domain.
- Information by UniProt
- DnaJ (Hsp40) homolog subfamily C member 8 antibody
- DnaJ homolog subfamily C member 8 antibody
- DNAJC 8 antibody
All lanes : Anti-DjC8 antibody (ab86138) at 0.04 µg/ml
Lane 1 : Whole cell lysate from HeLa cells at 50 µg
Lane 2 : Whole cell lysate from HeLa cells at 15 µg
Lane 3 : Whole cell lysate from HeLa cells at 5 µg
Lane 4 : Whole cell lysate from 293T cells at 50 µg
Developed using the ECL technique.
Predicted band size: 30 kDa
Observed band size: 36 kDa why is the actual band size different from the predicted?
Additional bands at: 18 kDa, 48 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
Detection of DjC8 in Immunoprecipitates of Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded) using ab86138 at 3 µg/mg lysate for IP (Lane 1) and at 1 µg/ml for subsequent Western blot detection. Lane 2 represents control IgG IP. Detection: Chemiluminescence with an exposure time of 3 seconds.
ab86138 has not yet been referenced specifically in any publications.