Product nameAnti-DKFZP434B168 antibody
See all DKFZP434B168 primary antibodies
DescriptionRabbit polyclonal to DKFZP434B168
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Horse, Chicken, Cow, Dog, Turkey, Pig, Chimpanzee, Ferret, Rhesus monkey, Gorilla, Orangutan
Synthetic peptide corresponding to Human DKFZP434B168 aa 850-900.
Database link: 25896
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.1% Sodium azide
Constituents: 0.021% PBS, 1.764% Sodium citrate, 1.815% Tris
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab17256 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/500 - 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.|
|WB||1/10000 - 1/25000. Detects a band of approximately 110 kDa.|
FunctionComponent of the Integrator complex, a complex involved in the small nuclear RNAs (snRNA) U1 and U2 transcription and in their 3'-box-dependent processing. The Integrator complex is associated with the C-terminal domain (CTD) of RNA polymerase II largest subunit (POLR2A) and is recruited to the U1 and U2 snRNAs genes. Plays a role in DNA damage response (DDR) signaling during the S phase.
Sequence similaritiesBelongs to the Integrator subunit 7 family.
Cellular localizationNucleus. Chromosome. Localizes to sites of DNA damage in a H2AX-independent manner.
- Information by UniProt
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue labeling DKFZP434B168 with ab17256 at 1/1000 (1µg/ml). Detection: DAB.
Lane 1 : Anti-DKFZP434B168 antibody (ab17256) at 1/25000 dilution
Lane 2 : Anti-DKFZP434B168 antibody (ab17256) at 1/10000 dilution
All lanes : Nuclear lysates prepared from Hela cells
Observed band size: 110 kDa why is the actual band size different from the predicted?
Sample: Nuclear extract (NE) from HeLa cells.
Antibody: ab17256 at 0.1 ug/ml. The band corresponding to DKFZP434B168 is arrowed at 110 kD. The identity of the other bands is unknown.
Detection: Chemiluminescence with an exposure time of 15 minutes.
We are unsure about the identity of other bands.
ab17256 has not yet been referenced specifically in any publications.