Overview

  • Product name

  • Description

    Rabbit polyclonal to DKK1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Fr, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (Human) from the internal domain of DKK1 conjugated to KLH

  • Positive control

    • Human uterus, endometrium.

Properties

Applications

Our Abpromise guarantee covers the use of ab61034 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-Fr 1/200.
IHC-P Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Steam slides in 0.01 M sodium citrate buffer, pH 6.0 at 99-100°C for 20 minutes. Remove from heat and let stand at room temperature in buffer for 20 minutes. Rinse in 1X TBS with Tween (TBST) for 1 minute at RT.

Target

  • Function

    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificity

    Placenta.
  • Sequence similarities

    Belongs to the dickkopf family.
  • Domain

    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • Dickkopf 1 antibody
    • Dickkopf 1 homolog antibody
    • Dickkopf 1 like antibody
    • Dickkopf homolog 1 antibody
    • Dickkopf like protein 1 antibody
    • Dickkopf related protein 1 antibody
    • Dickkopf WNT signaling pathway inhibitor 1 antibody
    • Dickkopf-1 antibody
    • Dickkopf-related protein 1 antibody
    • DKK 1 antibody
    • Dkk-1 antibody
    • Dkk1 antibody
    • DKK1_HUMAN antibody
    • hDkk 1 antibody
    • hDkk-1 antibody
    • SK antibody
    see all

Images

  • ab61034 at 10µg/ml staining formalin fixed paraffin embedded Human Uterus (endometrium).

  • ab61034 staining DKK1 in mouse embryonic (E16.5) craniofacial tissue by IHC-Fr (paraformaldehyde-fixed frozen sections).  Paraformaldehyde fixed issue samples were permealized in 0.1% Triton X-100, blocked with 5% serum for 15 minutes at 25 °C. The sample was incubated with primary antibody dilution 1/100 in PBS at 4°C for 24 hours. An Alexa Fluor®  conjugated Goat polyclonal to rabbit IgG (dilution 1/200)  was used as secondary antibody.

    See Abreview

References

This product has been referenced in:

  • Gavriilidis P  et al. Targeting hepatocarcinogenesis model in C56BL6 mice with pan-aurora kinase inhibitor Danusertib. J Cancer 9:914-922 (2018). Read more (PubMed: 29581770) »
  • Grinchuk OV  et al. Tumor-adjacent tissue co-expression profile analysis reveals pro-oncogenic ribosomal gene signature for prognosis of resectable hepatocellular carcinoma. Mol Oncol 12:89-113 (2018). IHC-P ; Human . Read more (PubMed: 29117471) »
See all 12 Publications for this product

Customer reviews and Q&As

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1-5 of 5 Abreviews

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C
Fixative
Paraformaldehyde

Ahmar Aziz

Verified customer

Submitted Jul 08 2016

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9, 20 min at 100C
Sample
Human Tissue sections (colon cancer)
Specification
colon cancer
Permeabilization
No
Fixative
Formaldehyde

Abcam user community

Verified customer

Submitted Dec 30 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, Dako, Citrate, Trypsin
Sample
Mouse Tissue sections (E10.5, E15.5 embryos)
Specification
E10.5, E15.5 embryos
Permeabilization
No
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Oct 17 2013

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Protein-Block Serum Free (DakoCytomation) as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Antigen retrieval step
Other
Sample
Mouse Tissue sections (mice bone cell)
Specification
mice bone cell
Permeabilization
No
Fixative
10% neutral-buffered formalin

Abcam user community

Verified customer

Submitted Jun 19 2013

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Head of Mouse Embryos E16.5)
Specification
Head of Mouse Embryos E16.5
Fixative
Paraformaldehyde
Permeabilization
Yes - Triton X-100 (0.1%)
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Gaskon Ibarretxe

Verified customer

Submitted Dec 02 2009

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