Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DKK1 antibody

Inconclusive
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA, Dako, Citrate, Trypsin
Sample
Mouse Tissue sections (E10.5, E15.5 embryos)
Specification
E10.5, E15.5 embryos
Permeabilization
No
Fixative
Paraformaldehyde

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% NGS
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Alexa-488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/1000

Additional data

Additional Notes
Resulting pattern inconclusive. Does not match known RNA patterns. Antigen Retrieval Method: 1x Dako-- Dilute 10x Dako solution. Pressure cook 10'. Allow to cool to RT. Wash in 200mM Glycine 10' at RT. Wash with 1x PBS. Block in 5% NGS 1 hr at RT. Incubate antibody 1:200 overnight at 4 degrees C. 4) Citrate-- 1:100 dilution stock solution Vector Cat# H3300. Pressure cook 10'. 5) 0.25% Trypsin-EDTA-- 20' at 37 degrees C. Attempted antibody dilutions: 1:100, 1:200, 1:500, 1:1000. Incubation conditions: RT 2 hours 4 degrees C, overnight.

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Submitted Oct 17 2013

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