Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DKK1 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Protein-Block Serum Free (DakoCytomation) as blocking agent for 10 minute(s) · Concentration: 0.25% · Temperature: RT°C
Antigen retrieval step
Other
Sample
Mouse Tissue sections (mice bone cell)
Specification
mice bone cell
Permeabilization
No
Fixative
10% neutral-buffered formalin

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100

Secondary antibody

Name
Non-Abcam antibody was used: Dako Anti-Rabbit IgG
Host species: Goat
Clonality: Monoclonal
Conjugation: Biotin
Dilution
1/50

Additional data

Additional Notes
The specificity of anti-DKK1 antibody has been demonstrated in osteocyte and bone marrow cells as indicated with red arrows. While no staining observed in negative control IgG. It should be noted that sample are decalification by 14% EDTA for 7 days at 4'C. Where other decalification can affect the proteinase K on epitope retrieval. And there are no staining can be seen without epitope retrieval.

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Submitted Jun 19 2013

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