Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-DKK1 antibody

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: EDTA pH9, 20 min at 100C
Sample
Human Tissue sections (colon cancer)
Specification
colon cancer
Permeabilization
No
Fixative
Formaldehyde

Other product details

Incubation time
20 minute(s) · Temperature: 25°C · Diluent: Dako diluent
Dilution
1/200

Secondary antibody

Name
Non-Abcam antibody was used: Leica Refine anti-mouse/rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer

Additional data

Additional Notes
various retrieval regimes tested (none, enzyme, citrate, EDTA) Automated on Leica Bond III

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Submitted Dec 30 2013

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