Anti-DKK1 antibody - C-terminal (ab188597)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-DKK1 antibody - C-terminal
See all DKK1 primary antibodies -
Description
Goat polyclonal to DKK1 - C-terminal -
Host species
Goat -
Tested applications
Suitable for: WB, ELISA, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat, Rabbit, Goat, Horse, Hamster, Cow, Dog, Pig, Monkey, Gorilla, Common marmoset, Bat, Platypus
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Immunogen
Synthetic peptide corresponding to Human DKK1 aa 250-262 (Cysteine residue).
Sequence:DHHQASNSSRLHT
Database link: O94907 -
Positive control
- Human placenta
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 99% Tris buffered saline, 0.5% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab188597 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 1 - 3 µg/ml. Predicted molecular weight: 27 kDa. | |
| ELISA | 1/128000. | |
| IHC-P | Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
Tissue specificity
Placenta. -
Sequence similarities
Belongs to the dickkopf family. -
Domain
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 504445 Cow
- Entrez Gene: 609600 Dog
- Entrez Gene: 102182190 Goat
- Entrez Gene: 101147272 Gorilla
- Entrez Gene: 101827192 Hamster
- Entrez Gene: 100071881 Horse
- Entrez Gene: 22943 Human
- Entrez Gene: 13380 Mouse
see all -
Alternative names
- Dickkopf 1 antibody
- Dickkopf 1 homolog antibody
- Dickkopf 1 like antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody - C-terminal (ab188597)Immunohistochemical analysis of formalin fixed paraffin embedded human placenta labeling DKK1 with ab188597 at 10 µg/ml
Datasheets and documents
References
This product has been referenced in:
- Workman A et al. The Wnt Signaling Pathway Is Differentially Expressed during the Bovine Herpesvirus 1 Latency-Reactivation Cycle: Evidence That Two Protein Kinases Associated with Neuronal Survival, Akt3 and BMPR2, Are Expressed at Higher Levels during Latency. J Virol 92:N/A (2018). Read more (PubMed: 29321317) »
- Himburg HA et al. Dickkopf-1 promotes hematopoietic regeneration via direct and niche-mediated mechanisms. Nat Med 23:91-99 (2017). Read more (PubMed: 27918563) »
