Recombinant
RabMAb

Recombinant Anti-DKK1 antibody [EPR4759] (ab109416)

Rabbit recombinant monoclonal DKK1 antibody [EPR4759]. Validated in WB, IHC and tested in Human. Cited in 12 publication(s). Independently reviewed in 1 review(s).

Reviews (1) Submit a question References (18)

Overview

  • Product name

    Anti-DKK1 antibody [EPR4759]
    See all DKK1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4759] to DKK1

  • Host species

    Rabbit

  • Specificity

    Although our internal data shows that ab109416 is not suitable to detect DKK1 in rats, ab109416 has been referenced in PMID 29299122 and 25469240 to work with rat derived cells. Please contact technical@abcam.com for further information.

  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt or ICC/IF

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Synthetic peptide within Human DKK1 aa 200 to the C-terminus. The exact sequence is proprietary.

  • Positive control

    • WB: HEK-293, Caco-2, A549, PC-3, NCI-H460, DU145 and SK-OV-3 whole cell lysates IHC-P: Human placenta and liver tissue

  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109416 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
IHC-P 1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

1/250.00000 - 1/500.00000
  • Application notes
    Is unsuitable for Flow Cyt or ICC/IF.

    • Target

    • Function

      Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

    • Tissue specificity

      Placenta.

    • Sequence similarities

      Belongs to the dickkopf family.

    • Domain

      The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

    • Cellular localization

      Secreted.
    • Information by UniProt

    • Database links

      • Alternative names

        • Dickkopf 1 antibody
        • Dickkopf 1 homolog antibody
        • Dickkopf 1 like antibody
        • Dickkopf homolog 1 antibody
        • Dickkopf like protein 1 antibody
        • Dickkopf related protein 1 antibody
        • Dickkopf WNT signaling pathway inhibitor 1 antibody
        • Dickkopf-1 antibody
        • Dickkopf-related protein 1 antibody
        • DKK 1 antibody
        • Dkk-1 antibody
        • Dkk1 antibody
        • DKK1_HUMAN antibody
        • hDkk 1 antibody
        • hDkk-1 antibody
        • SK antibody
        see all

      Images

      • Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
        Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
        All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/10000 dilution

        Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
        Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 29 kDa
        Observed band size: 29-40 kDa
        why is the actual band size different from the predicted?


        Exposure time: 180 seconds


        Blocking/Diluting buffer and concentration 5% NFDM/TBST.

        HEK-293 and 293T cell are weak expressed cell lines for DKK1 protein (PMID: 18502762,  PMID: 15378020).

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling DKK1 with Purified ab109416 at 1:800 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain
      • Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
        Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
        Anti-DKK1 antibody [EPR4759] (ab109416) at 1/1000 dilution + His-Tagged human DKK1 (aa 32 to 266) recombinant protein, 100ng

        Secondary
        Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 29 kDa



        Blocking/Diluting buffer and concentration 5% NFDM /TBST

        Exposure time: 5.5 seconds

         

      • Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
        Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)
        All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution (purified)

        Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
        Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
        Lane 3 : NCI-H460 (Human Large Cell Lung Cancer epithelial cell) whole cell lysates
        Lane 4 : DU145 (Human prostate carcinoma epithelial cell) whole cell lysates
        Lane 5 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysates

        Lysates/proteins at 20 µg per lane.

        Secondary
        All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

        Predicted band size: 29 kDa



        Blocking and diluting buffer: 5% NFDM/TBST.

        The smear bands detected by ab109416 are similar to those reported by the papers (PMID: 18546262, 28090290, 24008318), which should be due to the glycosylation of DKK1. 

      • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)
        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)

        Immunohistochemical analysis of paraffin-embedded Human placenta tissue using 1/100 unpurified ab109416

      References

      This product has been referenced in:

      • Wang W  et al. miR-410 acts as an oncogene in colorectal cancer cells by targeting dickkopf-related protein 1 via the Wnt/ß-catenin signaling pathway. Oncol Lett 17:807-814 (2019). Read more (PubMed: 30655833) »
      • Mehdinejadiani S  et al. Effects of letrozole and clomiphene citrate on Wnt signaling pathway in endometrium of polycystic ovarian syndrome and healthy women†. Biol Reprod 100:641-648 (2019). Read more (PubMed: 30184105) »
      See all 18 Publications for this product

      Publishing research using ab109416? Please let us know so that we can cite the reference in this datasheet.

      Customer reviews and Q&As

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      Western blot abreview for Anti-DKK1 antibody [EPR4759]

       Below Average
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (HUVEC)
      Loading amount
      20 µg
      Specification
      HUVEC
      Gel Running Conditions
      Reduced Denaturing (8% gel)
      Blocking step
      BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 25°C
      Read More

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      Submitted Nov 16 2011

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