Recombinant Anti-DKK1 antibody [EPR4759] (ab109416)
![Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)](https://www.abcam.com/ps/products/109/ab109416/Images/ab109416-472629-anti-dkk1-antibody-epr4759-western-blot-caco2-pc3-u2-os-molt4.jpg "Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4759] to DKK1
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
Related conjugates and formulations
Overview
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Product name
Anti-DKK1 antibody [EPR4759]
See all DKK1 primary antibodies -
Description
Rabbit monoclonal [EPR4759] to DKK1 -
Host species
Rabbit -
Specificity
Although our internal data shows that ab109416 is not suitable to detect DKK1 in rats, ab109416 has been referenced in PMID 29299122 and 25469240 to work with rat derived cells. Please contact technical@abcam.com for further information. -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or ICC/IF -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: HEK-293, Caco-2, U-2 OS, MOLT4, A549, PC-3, NCI-H460, DU145 and SK-OV-3 whole cell lysates IHC-P: Human placenta and liver tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4759 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109416 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
|
| IHC-P |
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
See IHC antigen retrieval protocols. 1/250.00000 - 1/500.00000 |
| Notes |
|---|
|
WB
1/1000 - 1/10000. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa). |
|
IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. See IHC antigen retrieval protocols. 1/250.00000 - 1/500.00000 |
Target
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Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
Tissue specificity
Placenta. -
Sequence similarities
Belongs to the dickkopf family. -
Domain
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 22943 Human
- Omim: 605189 Human
- SwissProt: O94907 Human
- Unigene: 40499 Human
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Alternative names
- Dickkopf 1 antibody
- Dickkopf 1 homolog antibody
- Dickkopf 1 like antibody
see all
Images
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Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution
Lane 1 : Caco-2 cell lysate
Lane 2 : PC-3 cell lysate
Lane 3 : U-2 OS cell lysate
Lane 4 : MOLT4 cell lysate
Lane 5 : HEK-293 cell lysate
Performed under reducing conditions.
Predicted band size: 29 kDa
Observed band size: 30-45 kDa why is the actual band size different from the predicted?False colour image of Western blot: Anti-DKK1 antibody [EPR4759] staining at 1/2000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. To generate this image, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween® 20 (TBS-T) before incubation with primary antibodies overnight at 4 °C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)](https://www.abcam.com/ps/products/109/ab109416/Images/ab109416-314302-anti-dkk1-antibody-epr4759-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling DKK1 with Purified ab109416 at 1:800 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain -
![Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)](https://www.abcam.com/ps/products/109/ab109416/Images/ab109416-338271-anti-dkk1-antibody-epr4759-western-blot.jpg)
Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/10000 dilution
Lane 1 : Caco-2 (Human colorectal adenocarcinoma epithelial cell) whole cell lysate
Lane 2 : HEK-293 (Human embryonic kidney epithelial cell) whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDa
Observed band size: 29-40 kDa why is the actual band size different from the predicted?
Exposure time: 180 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST.
HEK-293 and 293T cell are weak expressed cell lines for DKK1 protein (PMID: 18502762, PMID: 15378020).
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![Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)](https://www.abcam.com/ps/products/109/ab109416/Images/ab109416-337286-anti-dkk1-antibody-epr4759-western-blot.png)
Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)Anti-DKK1 antibody [EPR4759] (ab109416) at 1/1000 dilution + His-Tagged human DKK1 (aa 32 to 266) recombinant protein, 100ng
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDaBlocking/Diluting buffer and concentration 5% NFDM /TBST
Exposure time: 5.5 seconds
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![Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)](https://www.abcam.com/ps/products/109/ab109416/Images/ab109416-314262-anti-dkk1-antibody-epr4759-western-blot.jpg)
Western blot - Anti-DKK1 antibody [EPR4759] (ab109416)All lanes : Anti-DKK1 antibody [EPR4759] (ab109416) at 1/2000 dilution (purified)
Lane 1 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 2 : PC-3 (Human prostate adenocarcinoma epithelial cell) whole cell lysates
Lane 3 : NCI-H460 (Human Large Cell Lung Cancer epithelial cell) whole cell lysates
Lane 4 : DU145 (Human prostate carcinoma epithelial cell) whole cell lysates
Lane 5 : SK-OV-3 (Human ovarian cancer epithelial cell) whole cell lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 29 kDaBlocking and diluting buffer: 5% NFDM/TBST.
The smear bands detected by ab109416 are similar to those reported by the papers (PMID: 18546262, 28090290, 24008318), which should be due to the glycosylation of DKK1. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)](https://www.abcam.com/ps/products/109/ab109416/Images/ab109416-122240-anti-dkk1-antibody-epr4759-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DKK1 antibody [EPR4759] (ab109416)Immunohistochemical analysis of paraffin-embedded Human placenta tissue using 1/100 unpurified ab109416.
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![Anti-DKK1 antibody [EPR4759] (ab109416)](https://www.abcam.com/ps/products/109/ab109416/Images/ab109416-8-benefits-of-recombinant-antibodies.png)
Anti-DKK1 antibody [EPR4759] (ab109416)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (34)
ab109416 has been referenced in 34 publications.
- Shailesh H et al. Protein arginine methyltransferase 5 (PRMT5) activates WNT/ß-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3. J Cell Mol Med 25:1583-1600 (2021). PubMed: 33462997
- Qi L et al. HomeoboxC6 promotes metastasis by orchestrating the DKK1/Wnt/ß-catenin axis in right-sided colon cancer. Cell Death Dis 12:337 (2021). PubMed: 33795652
- Zhao Y et al. Andrographis overcomes 5-fluorouracil-associated chemoresistance through inhibition of DKK1 in colorectal cancer. Carcinogenesis 42:814-825 (2021). PubMed: 33822896
- Zhou G et al. The regulation effect of WNT-RAS signaling in hypothalamic paraventricular nucleus on renal fibrosis. J Nephrol 33:289-297 (2020). PubMed: 31392659
- Bak DH et al. Protective effects of human umbilical cord blood-derived mesenchymal stem cells against dexamethasone-induced apoptotic cell death in hair follicles. Int J Mol Med 45:556-568 (2020). PubMed: 31894311