Recombinant
RabMAb

Recombinant Anti-DKK1 antibody [EPR4759] - Low endotoxin, Azide free (ab221779)

Overview

  • Product name

    Anti-DKK1 antibody [EPR4759] - Low endotoxin, Azide free
    See all DKK1 primary antibodies
  • Description

    Rabbit monoclonal [EPR4759] to DKK1 - Low endotoxin, Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC/IF or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    A synthetic peptide corresponding to residues in Human DKK1

  • Positive control

    • JAR, HepG2, HeLa, and NCCIT cell lysates; Human placenta tissue; HeLa cells.
  • General notes

    ab221779 is a carrier-free antibody designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our Low endotoxin, azide-free formats have low endotoxin level (≤ 1 EU/ml, determined by the LAL assay) and are free from azide, to achieve consistent experimental results in functional assays.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab221779 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 38 kDa (predicted molecular weight: 29 kDa).
IHC-P Use at an assay dependent concentration.

antigen retrieval is recommended.

  • Application notes
    Is unsuitable for Flow Cyt,ICC/IF or IP.
  • Target

    • Function

      Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
    • Tissue specificity

      Placenta.
    • Sequence similarities

      Belongs to the dickkopf family.
    • Domain

      The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
    • Cellular localization

      Secreted.
    • Information by UniProt
    • Database links

    • Alternative names

      • Dickkopf 1 antibody
      • Dickkopf 1 homolog antibody
      • Dickkopf 1 like antibody
      • Dickkopf homolog 1 antibody
      • Dickkopf like protein 1 antibody
      • Dickkopf related protein 1 antibody
      • Dickkopf WNT signaling pathway inhibitor 1 antibody
      • Dickkopf-1 antibody
      • Dickkopf-related protein 1 antibody
      • DKK 1 antibody
      • Dkk-1 antibody
      • Dkk1 antibody
      • DKK1_HUMAN antibody
      • hDkk 1 antibody
      • hDkk-1 antibody
      • SK antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human liver tissue sections labeling DKK1 with Purified ab109416 at 1:800 dilution (1.04 µg/ml). Heat mediated antigen retrieval was performed using using ab93684 (Tris/EDTA buffer, pH 9.0)ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody.Negative control:PBS instead of the primary antibody.Hematoxylinwas used as a counterstain

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109416).

    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue using 1/100 unpurified ab109416

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109416).

    References

    This product has been referenced in:

    • Li Y  et al. Minocycline protects against hepatic ischemia/reperfusion injury in a rat model. Biomed Rep 3:19-24 (2015). WB ; Rat . Read more (PubMed: 25469240) »
    • Lyros O  et al. Dickkopf-1, the Wnt antagonist, is induced by acidic pH and mediates epithelial cellular senescence in human reflux esophagitis. Am J Physiol Gastrointest Liver Physiol 306:G557-74 (2014). IF ; Human . Read more (PubMed: 24481601) »
    See all 4 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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