Key features and details
- Rabbit polyclonal to DKK2
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-DKK2 antibody
See all DKK2 primary antibodies
DescriptionRabbit polyclonal to DKK2
SpecificityDKK2 fragments of ~55 kDa and ~28 kDa can also be detected in rat kidney tissue lysate
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide, surrounding amino acid 241 of Mouse DKK2
- Lysate from Jurkat cells, 3T3 cells and rat kidney tissue
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.01% Thimerosal (merthiolate)
Constituents: 0.5% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
Purification notesab95274 is affinity purified.
Our Abpromise guarantee covers the use of ab95274 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 28 kDa.|
FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Tissue specificityExpressed in heart, brain, skeletal muscle and lung.
Sequence similaritiesBelongs to the dickkopf family.
DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
modificationsMay be proteolytically processed by a furin-like protease.
- Information by UniProt
- Dickkopf 2 antibody
- Dickkopf gene 2 antibody
- Dickkopf homolog 2 antibody
Lane 1 : Anti-DKK2 antibody (ab95274) at 4 µg/ml
Lanes 2-4 : Anti-DKK2 antibody (ab95274) at 4 µg/ml (in 1X TBS/T with 5% nonfat milk, overnight at 4C.)
Lanes 1-2 : Jurkat cell lysate
Lane 3 : 3T3 cell lysate
Lane 4 : Rat kidney tissue lysate
All lanes : Anti-rabbit conjugated secondary antibody
Predicted band size: 28 kDa
ab95274 has been referenced in 2 publications.
- Yin GN et al. Pericyte-Derived Dickkopf2 Regenerates Damaged Penile Neurovasculature Through an Angiopoietin-1-Tie2 Pathway. Diabetes 67:1149-1161 (2018). PubMed: 29559443
- Sopko R et al. Engineering potent long-acting variants of the Wnt inhibitor DKK2. Protein Eng Des Sel 30:359-372 (2017). WB . PubMed: 28180900