The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/100 - 1/500. Detects a band of approximately 28 kDa (predicted molecular weight: 28 kDa).
Use at an assay dependent concentration.
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Expressed in heart, brain, skeletal muscle and lung.
Belongs to the dickkopf family.
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
May be proteolytically processed by a furin-like protease.
Mu J et al. Dickkopf-related protein 2 induces G0/G1 arrest and apoptosis through suppressing Wnt/ß-catenin signaling and is frequently methylated in breast cancer. Oncotarget8:39443-39459 (2017).
WB, IF, IHC
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