Overview

  • Product name
  • Description
    Rabbit polyclonal to Dkk3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-Pmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human Dkk3.
    Database link: Q9UBP4

  • Positive control
    • Mouse brain extract.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.3
    Preservative: 0.02% Sodium azide
    Constituents: 49% PBS, 50% Glycerol
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Clonality
    Polyclonal
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab187532 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 38 kDa.
IHC-P 1/50 - 1/200.

Target

  • Function
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificity
    Highest expression in heart, brain, and spinal cord.
  • Sequence similarities
    Belongs to the dickkopf family.
  • Domain
    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dickkopf 3 antibody
    • Dickkopf 3 homolog (Xenopus laevis) antibody
    • Dickkopf 3 homolog antibody
    • Dickkopf homolog 3 antibody
    • Dickkopf related protein 3 antibody
    • Dickkopf related protein 3 Precursor antibody
    • Dickkopf WNT signaling pathway inhibitor 3 antibody
    • Dickkopf, Xenopus, homolog of, 3 antibody
    • Dickkopf-3 antibody
    • Dickkopf-related protein 3 antibody
    • Dickkopf3 antibody
    • DKK 3 antibody
    • Dkk-3 antibody
    • DKK3 antibody
    • DKK3_HUMAN antibody
    • hDkk 3 antibody
    • hDkk-3 antibody
    • hDkk3 antibody
    • Regulated in glioma antibody
    • REIC antibody
    • RIG antibody
    • RIG-like 5-6 antibody
    • RIG-like 7-1 antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human thyroid cancer tissue labelling Dkk3 with ab187532 at 1/100. Magnification: 200x.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human endometrial cancer tissue labelling Dkk3 with ab187532 at 1/100. Magnification: 200x.
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast cancer tissue labelling Dkk3 with ab187532 at 1/100. Magnification: 200x.
  • Anti-Dkk3 antibody (ab187532) at 1/500 dilution + Mouse brain extract

    Predicted band size: 38 kDa

References

This product has been referenced in:
  • Wu Q  et al. MicroRNA-92b promotes cell proliferation and invasion in osteosarcoma by directly targeting Dickkopf-related protein 3. Exp Ther Med 15:173-181 (2018). Read more (PubMed: 29250147) »
  • Park NS  et al. Meridianin C inhibits the growth of YD-10B human tongue cancer cells through macropinocytosis and the down-regulation of Dickkopf-related protein-3. J Cell Mol Med 22:5833-5846 (2018). Read more (PubMed: 30246484) »
See all 2 Publications for this product

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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