Western blot abreview for Anti-Dkk3 antibody

Good
Abreviews
Application
Western blot
Sample
Human Tissue lysate - other (Cerebrospinal fluid (CSF))
Loading amount
3 µg
Specification
Cerebrospinal fluid (CSF)
Gel Running Conditions
Reduced Denaturing (12% SDS)
Blocking step
Milk as blocking agent for 17 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: + 4°C

Other product details

Dilution
1/500
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 22°C · Diluent: 5% milk in TBS-T

Secondary antibody

Name
Non-Abcam antibody was used: Donkey anti-goat IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/8000

Detection

Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: ~ 60 kDa

Additional data

Additional Notes
Sample is body fluid (CSF).

Ms. Christiane Rewerts

Verified customer

Submitted Jun 04 2009

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