Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dkk3 antibody

Excellent
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Human MDA-MB 436 cells grown subcutaneously in mou)
Specification
Human MDA-MB 436 cells grown subcutaneously in mou
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate-EDTA (pH6)
Permeabilization
No
Blocking step
casein as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 22°C

Other product details

Dilution
1/75
Incubation time
30 minute(s) · Temperature: 22°C · Diluent: 10% casein

Secondary antibody

Name
Non-Abcam antibody was used: rabbit anti-goat biotinylated secondary antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/200

Abcam user community

Verified customer

Submitted Oct 27 2009

Sign up