Western blot abreview for Anti-Dkk3 antibody

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Human MDA-MB-436 Breast Cancer Cells)
Loading amount
3 µg
Specification
Human MDA-MB-436 Breast Cancer Cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
1xCasein as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 22°C

Other product details

Dilution
1/250
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: Signal Boost

Secondary antibody

Name
Non-Abcam antibody was used: Anti-goat HRP linked secondary
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/20000

Detection

Detection method
Supersignal West Dura
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 65 kDa

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Submitted Apr 27 2010

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