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    dkk3-antibody-epr15611-ab186409.pdf

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Signal Transduction Adapters Transmembrane
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RecombinantRabMAb

Recombinant Anti-Dkk3 antibody [EPR15611] (ab186409)

  • Datasheet
  • Certificate of Compliance
Submit a review Submit a question References (10)

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Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
  • Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (ab186409)
  • Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
  • Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)
  • Anti-Dkk3 antibody [EPR15611] (ab186409)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15611] to Dkk3
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Carrier Free

You may also be interested in

Primary
Product image
Anti-PGD antibody [EPR6565] (ab129199)
Protein
Recombinant human Dkk3 protein (ab186078)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-Dkk3 antibody [EPR15611]
    See all Dkk3 primary antibodies
  • Description

    Rabbit monoclonal [EPR15611] to Dkk3
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • Human fetal brain, heart and kidney lysates; HepG2 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR15611
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Adapters
    • Transmembrane
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Secreted

Associated products

  • Alternative Versions

    • Alexa Fluor® 488 Anti-Dkk3 antibody [EPR15611] (ab201262)
    • Anti-Dkk3 antibody [EPR15611] - BSA and Azide free (ab232657)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant human Dkk3 protein (ab186078)
  • Related Products

    • Recombinant Human Dkk3 (mutated R335G) protein (ab155717)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab186409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 38 kDa).
ICC/IF
1/100.
IP
1/50 - 1/70.
Notes
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 38 kDa).
ICC/IF
1/100.
IP
1/50 - 1/70.

Target

  • Function

    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificity

    Highest expression in heart, brain, and spinal cord.
  • Sequence similarities

    Belongs to the dickkopf family.
  • Domain

    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Post-translational
    modifications

    N- and O-glycosylated.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q9UBP4 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 27122 Human
    • Entrez Gene: 50781 Mouse
    • Entrez Gene: 171548 Rat
    • Omim: 605416 Human
    • SwissProt: Q9UBP4 Human
    • SwissProt: Q9QUN9 Mouse
    • Unigene: 292156 Human
    • Unigene: 55143 Mouse
    • Alternative names

      • Dickkopf 3 antibody
      • Dickkopf 3 homolog (Xenopus laevis) antibody
      • Dickkopf 3 homolog antibody
      • Dickkopf homolog 3 antibody
      • Dickkopf related protein 3 antibody
      • Dickkopf related protein 3 Precursor antibody
      • Dickkopf WNT signaling pathway inhibitor 3 antibody
      • Dickkopf, Xenopus, homolog of, 3 antibody
      • Dickkopf-3 antibody
      • Dickkopf-related protein 3 antibody
      • Dickkopf3 antibody
      • DKK 3 antibody
      • Dkk-3 antibody
      • DKK3 antibody
      • DKK3_HUMAN antibody
      • hDkk 3 antibody
      • hDkk-3 antibody
      • hDkk3 antibody
      • Regulated in glioma antibody
      • REIC antibody
      • RIG antibody
      • RIG-like 5-6 antibody
      • RIG-like 7-1 antibody
      see all

    Images

    • Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/10000 dilution + Human fetal brain lysate at 20 µg

      Secondary
      Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

      Predicted band size: 38 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (ab186409)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dkk3 with ab186409 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    • Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      All lanes : Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/5000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 38 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)

      Western blot analysis of Dkk3 in Human fetal brain lysate immunoprecipitated with ab186409 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal brain lysate.

      Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    • Anti-Dkk3 antibody [EPR15611] (ab186409)
      Anti-Dkk3 antibody [EPR15611] (ab186409)

    Protocols

    • Western blot protocols
    • Immunoprecipitation protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (10)

    Publishing research using ab186409? Please let us know so that we can cite the reference in this datasheet.

    ab186409 has been referenced in 10 publications.

    • Shailesh H  et al. Protein arginine methyltransferase 5 (PRMT5) activates WNT/ß-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3. J Cell Mol Med 25:1583-1600 (2021). PubMed: 33462997
    • Xu Y  et al. DKK3 attenuates JNK and AP-1 induced inflammation via Kremen-1 and DVL-1 in mice following intracerebral hemorrhage. J Neuroinflammation 17:130 (2020). PubMed: 32331523
    • Li Y  et al. Long noncoding RNA LINC00261 suppresses prostate cancer tumorigenesis through upregulation of GATA6-mediated DKK3. Cancer Cell Int 20:474 (2020). PubMed: 33013201
    • Yan L  et al. Inhibition of SMYD2 suppresses tumor progression by down-regulating microRNA-125b and attenuates multi-drug resistance in renal cell carcinoma. Theranostics 9:8377-8391 (2019). PubMed: 31754403
    • Katase N  et al. DKK3 knockdown confers negative effects on the malignant potency of head and neck squamous cell carcinoma cells via the PI3K/Akt and MAPK signaling pathways. Int J Oncol 54:1021-1032 (2019). PubMed: 30569110
    • Yu H  et al. MiR-92a modulates proliferation, apoptosis, migration, and invasion of osteosarcoma cell lines by targeting Dickkopf-related protein 3. Biosci Rep 39:N/A (2019). PubMed: 30926679
    • Zhu B  et al. Array profiling reveals contribution of Cthrc1 to growth of the denervated rat urinary bladder. Am J Physiol Renal Physiol 314:F893-F905 (2018). PubMed: 29357417
    • Leonard JL  et al. The Dkk3 gene encodes a vital intracellular regulator of cell proliferation. PLoS One 12:e0181724 (2017). PubMed: 28738084
    • Luo S  et al. MicroRNA-92a promotes cell viability and invasion in cervical cancer via directly targeting Dickkopf-related protein 3. Exp Ther Med 14:1227-1234 (2017). PubMed: 28810582
    • McCullough KM  et al. Molecular characterization of Thy1 expressing fear-inhibiting neurons within the basolateral amygdala. Nat Commun 7:13149 (2016). IHC-FrFl ; Mouse . PubMed: 27767183

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