Recombinant Anti-Dkk3 antibody [EPR15611] (ab186409)
- Datasheet
- References (4)
- Protocols
Overview
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Product nameAnti-Dkk3 antibody [EPR15611]
See all Dkk3 primary antibodies -
DescriptionRabbit monoclonal [EPR15611] to Dkk3
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Host speciesRabbit
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Tested applicationsSuitable for: WB, ICC/IF, IPmore details
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Species reactivityReacts with: Mouse, Rat, Human
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Immunogen
Recombinant fragment within Human Dkk3 aa 250 to the C-terminus. The exact sequence is proprietary.
Database link: Q9UBP4 -
Positive control
- Human fetal brain, heart and kidney lysates; HepG2 cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR15611
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab186409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 38 kDa). | |
| ICC/IF | 1/100. | |
| IP | 1/50 - 1/70. |
Target
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FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
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Tissue specificityHighest expression in heart, brain, and spinal cord.
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Sequence similaritiesBelongs to the dickkopf family.
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DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
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Post-translational
modificationsN- and O-glycosylated. -
Cellular localizationSecreted.
- Information by UniProt
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Database links
- Entrez Gene: 27122 Human
- Entrez Gene: 50781 Mouse
- Entrez Gene: 171548 Rat
- Omim: 605416 Human
- SwissProt: Q9UBP4 Human
- SwissProt: Q9QUN9 Mouse
- Unigene: 292156 Human
- Unigene: 55143 Mouse
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Alternative names
- Dickkopf 3 antibody
- Dickkopf 3 homolog (Xenopus laevis) antibody
- Dickkopf 3 homolog antibody
see all
Images
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![Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)](https://www.abcam.com/ps/products/186/ab186409/Images/ab186409-220906-ab186409a.jpg)
Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/10000 dilution + Human fetal brain lysate at 20 µg
Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (ab186409)](https://www.abcam.com/ps/products/186/ab186409/Images/ab186409-220904-ab186409c.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (ab186409)Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dkk3 with ab186409 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
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![Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)](https://www.abcam.com/ps/products/186/ab186409/Images/ab186409-220905-ab186409b.jpg)
Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)All lanes : Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/5000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)](https://www.abcam.com/ps/products/186/ab186409/Images/ab186409-220903-ab186409d.jpg)
Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)Western blot analysis of Dkk3 in Human fetal brain lysate immunoprecipitated with ab186409 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal brain lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
Protocols
Datasheets and documents
References
This product has been referenced in:
- Katase N et al. DKK3 knockdown confers negative effects on the malignant potency of head and neck squamous cell carcinoma cells via the PI3K/Akt and MAPK signaling pathways. Int J Oncol 54:1021-1032 (2019). Read more (PubMed: 30569110) »
- Leonard JL et al. The Dkk3 gene encodes a vital intracellular regulator of cell proliferation. PLoS One 12:e0181724 (2017). Read more (PubMed: 28738084) »
