Recombinant Anti-Dkk3 antibody [EPR15611] (ab186409)
![Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)](https://www.abcam.com/ps/products/186/ab186409/Images/ab186409-220906-anti-dkk3-antibody-epr15611-western-blot.jpg "Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15611] to Dkk3
- Suitable for: WB, ICC/IF, IP
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Dkk3 antibody [EPR15611]
See all Dkk3 primary antibodies -
Description
Rabbit monoclonal [EPR15611] to Dkk3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, ICC/IF, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat
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Immunogen
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, heart and kidney lysates; HepG2 cells.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15611 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab186409 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 38 kDa).
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| ICC/IF |
1/100.
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| IP |
1/50 - 1/70.
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| Notes |
|---|
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WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 38 kDa). |
|
ICC/IF
1/100. |
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IP
1/50 - 1/70. |
Target
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Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
Tissue specificity
Highest expression in heart, brain, and spinal cord. -
Sequence similarities
Belongs to the dickkopf family. -
Domain
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 27122 Human
- Entrez Gene: 50781 Mouse
- Entrez Gene: 171548 Rat
- Omim: 605416 Human
- SwissProt: Q9UBP4 Human
- SwissProt: Q9QUN9 Mouse
- Unigene: 292156 Human
- Unigene: 55143 Mouse
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Alternative names
- Dickkopf 3 antibody
- Dickkopf 3 homolog (Xenopus laevis) antibody
- Dickkopf 3 homolog antibody
see all
Images
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Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/10000 dilution + Human fetal brain lysate at 20 µg
Secondary
Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (ab186409)](https://www.abcam.com/ps/products/186/ab186409/Images/ab186409-220904-anti-dkk3-antibody-epr15611-immunofluorescence.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (ab186409)Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dkk3 with ab186409 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).
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![Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)](https://www.abcam.com/ps/products/186/ab186409/Images/ab186409-220905-anti-dkk3-antibody-epr15611-western-blot.jpg)
Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)All lanes : Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/5000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal heart lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
Predicted band size: 38 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted? -
![Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)](https://www.abcam.com/ps/products/186/ab186409/Images/ab186409-220903-anti-dkk3-antibody-epr15611-immunoprecipitation.jpg)
Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)Western blot analysis of Dkk3 in Human fetal brain lysate immunoprecipitated with ab186409 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal brain lysate.
Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.
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![Anti-Dkk3 antibody [EPR15611] (ab186409)](https://www.abcam.com/ps/products/186/ab186409/Images/ab186409-5-benefits-of-recombinant-antibodies.png)
Anti-Dkk3 antibody [EPR15611] (ab186409)
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (10)
ab186409 has been referenced in 10 publications.
- Shailesh H et al. Protein arginine methyltransferase 5 (PRMT5) activates WNT/ß-catenin signalling in breast cancer cells via epigenetic silencing of DKK1 and DKK3. J Cell Mol Med 25:1583-1600 (2021). PubMed: 33462997
- Xu Y et al. DKK3 attenuates JNK and AP-1 induced inflammation via Kremen-1 and DVL-1 in mice following intracerebral hemorrhage. J Neuroinflammation 17:130 (2020). PubMed: 32331523
- Li Y et al. Long noncoding RNA LINC00261 suppresses prostate cancer tumorigenesis through upregulation of GATA6-mediated DKK3. Cancer Cell Int 20:474 (2020). PubMed: 33013201
- Yan L et al. Inhibition of SMYD2 suppresses tumor progression by down-regulating microRNA-125b and attenuates multi-drug resistance in renal cell carcinoma. Theranostics 9:8377-8391 (2019). PubMed: 31754403
- Katase N et al. DKK3 knockdown confers negative effects on the malignant potency of head and neck squamous cell carcinoma cells via the PI3K/Akt and MAPK signaling pathways. Int J Oncol 54:1021-1032 (2019). PubMed: 30569110
- Yu H et al. MiR-92a modulates proliferation, apoptosis, migration, and invasion of osteosarcoma cell lines by targeting Dickkopf-related protein 3. Biosci Rep 39:N/A (2019). PubMed: 30926679
- Zhu B et al. Array profiling reveals contribution of Cthrc1 to growth of the denervated rat urinary bladder. Am J Physiol Renal Physiol 314:F893-F905 (2018). PubMed: 29357417
- Leonard JL et al. The Dkk3 gene encodes a vital intracellular regulator of cell proliferation. PLoS One 12:e0181724 (2017). PubMed: 28738084
- Luo S et al. MicroRNA-92a promotes cell viability and invasion in cervical cancer via directly targeting Dickkopf-related protein 3. Exp Ther Med 14:1227-1234 (2017). PubMed: 28810582
- McCullough KM et al. Molecular characterization of Thy1 expressing fear-inhibiting neurons within the basolateral amygdala. Nat Commun 7:13149 (2016). IHC-FrFl ; Mouse . PubMed: 27767183
