Recombinant
RabMAb

Recombinant Anti-Dkk3 antibody [EPR15611] (ab186409)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR15611] to Dkk3
  • Suitable for: WB, ICC/IF, IP
  • Reacts with: Mouse, Rat, Human

Overview

  • Product name

    Anti-Dkk3 antibody [EPR15611]
    See all Dkk3 primary antibodies

  • Description

    Rabbit monoclonal [EPR15611] to Dkk3

  • Host species

    Rabbit

  • Tested applications

    Suitable for: WB, ICC/IF, IPmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Recombinant fragment within Human Dkk3 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UBP4

  • Positive control

    • Human fetal brain, heart and kidney lysates; HepG2 cells.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...

  • Purity

    Protein A purified

  • Clonality

    Monoclonal

  • Clone number

    EPR15611

  • Isotype

    IgG

  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 38 kDa).
ICC/IF 1/100.
IP 1/50 - 1/70.

Target

  • Function

    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.

  • Tissue specificity

    Highest expression in heart, brain, and spinal cord.

  • Sequence similarities

    Belongs to the dickkopf family.

  • Domain

    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.

  • Post-translational
    modifications

    N- and O-glycosylated.

  • Cellular localization

    Secreted.
  • Information by UniProt

  • Database links

    • Alternative names

      • Dickkopf 3 antibody
      • Dickkopf 3 homolog (Xenopus laevis) antibody
      • Dickkopf 3 homolog antibody
      • Dickkopf homolog 3 antibody
      • Dickkopf related protein 3 antibody
      • Dickkopf related protein 3 Precursor antibody
      • Dickkopf WNT signaling pathway inhibitor 3 antibody
      • Dickkopf, Xenopus, homolog of, 3 antibody
      • Dickkopf-3 antibody
      • Dickkopf-related protein 3 antibody
      • Dickkopf3 antibody
      • DKK 3 antibody
      • Dkk-3 antibody
      • DKK3 antibody
      • DKK3_HUMAN antibody
      • hDkk 3 antibody
      • hDkk-3 antibody
      • hDkk3 antibody
      • Regulated in glioma antibody
      • REIC antibody
      • RIG antibody
      • RIG-like 5-6 antibody
      • RIG-like 7-1 antibody
      see all

    Images

    • Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/10000 dilution + Human fetal brain lysate at 20 µg

      Secondary
      Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

      Predicted band size: 38 kDa
      Observed band size: 60 kDa
      why is the actual band size different from the predicted?

    • Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Immunocytochemistry/ Immunofluorescence - Anti-Dkk3 antibody [EPR15611] (ab186409)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dkk3 with ab186409 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

    • Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Western blot - Anti-Dkk3 antibody [EPR15611] (ab186409)
      All lanes : Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/5000 dilution

      Lane 1 : Human fetal kidney lysate
      Lane 2 : Human fetal heart lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

      Predicted band size: 38 kDa
      Observed band size: 60 kDa why is the actual band size different from the predicted?

    • Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)
      Immunoprecipitation - Anti-Dkk3 antibody [EPR15611] (ab186409)

      Western blot analysis of Dkk3 in Human fetal brain lysate immunoprecipitated with ab186409 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal brain lysate.

      Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

    References

    Publishing research using ab186409? Please let us know so that we can cite the reference in this datasheet.

    ab186409 has been referenced in 8 publications.

    • Xu Y  et al. DKK3 attenuates JNK and AP-1 induced inflammation via Kremen-1 and DVL-1 in mice following intracerebral hemorrhage. J Neuroinflammation 17:130 (2020). PubMed: 32331523
    • Yan L  et al. Inhibition of SMYD2 suppresses tumor progression by down-regulating microRNA-125b and attenuates multi-drug resistance in renal cell carcinoma. Theranostics 9:8377-8391 (2019). PubMed: 31754403
    • Katase N  et al. DKK3 knockdown confers negative effects on the malignant potency of head and neck squamous cell carcinoma cells via the PI3K/Akt and MAPK signaling pathways. Int J Oncol 54:1021-1032 (2019). PubMed: 30569110
    • Yu H  et al. MiR-92a modulates proliferation, apoptosis, migration, and invasion of osteosarcoma cell lines by targeting Dickkopf-related protein 3. Biosci Rep 39:N/A (2019). PubMed: 30926679
    • Zhu B  et al. Array profiling reveals contribution of Cthrc1 to growth of the denervated rat urinary bladder. Am J Physiol Renal Physiol 314:F893-F905 (2018). PubMed: 29357417
    • Leonard JL  et al. The Dkk3 gene encodes a vital intracellular regulator of cell proliferation. PLoS One 12:e0181724 (2017). PubMed: 28738084
    • Luo S  et al. MicroRNA-92a promotes cell viability and invasion in cervical cancer via directly targeting Dickkopf-related protein 3. Exp Ther Med 14:1227-1234 (2017). PubMed: 28810582
    • McCullough KM  et al. Molecular characterization of Thy1 expressing fear-inhibiting neurons within the basolateral amygdala. Nat Commun 7:13149 (2016). IHC-FrFl ; Mouse . PubMed: 27767183

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