Overview

  • Product name
    Anti-Dkk3 antibody [EPR15611]
    See all Dkk3 primary antibodies
  • Description
    Rabbit monoclonal [EPR15611] to Dkk3
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, ICC/IF, IPmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment within Human Dkk3 aa 250 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9UBP4

  • Positive control
    • Human fetal brain, heart and kidney lysates; HepG2 cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity
    Protein A purified
  • Clonality
    Monoclonal
  • Clone number
    EPR15611
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab186409 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 38 kDa).
ICC/IF 1/100.
IP 1/50 - 1/70.

Target

  • Function
    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificity
    Highest expression in heart, brain, and spinal cord.
  • Sequence similarities
    Belongs to the dickkopf family.
  • Domain
    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Post-translational
    modifications
    N- and O-glycosylated.
  • Cellular localization
    Secreted.
  • Information by UniProt
  • Database links
  • Alternative names
    • Dickkopf 3 antibody
    • Dickkopf 3 homolog (Xenopus laevis) antibody
    • Dickkopf 3 homolog antibody
    • Dickkopf homolog 3 antibody
    • Dickkopf related protein 3 antibody
    • Dickkopf related protein 3 Precursor antibody
    • Dickkopf WNT signaling pathway inhibitor 3 antibody
    • Dickkopf, Xenopus, homolog of, 3 antibody
    • Dickkopf-3 antibody
    • Dickkopf-related protein 3 antibody
    • Dickkopf3 antibody
    • DKK 3 antibody
    • Dkk-3 antibody
    • DKK3 antibody
    • DKK3_HUMAN antibody
    • hDkk 3 antibody
    • hDkk-3 antibody
    • hDkk3 antibody
    • Regulated in glioma antibody
    • REIC antibody
    • RIG antibody
    • RIG-like 5-6 antibody
    • RIG-like 7-1 antibody
    see all

Images

  • Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/10000 dilution + Human fetal brain lysate at 20 µg

    Secondary
    Goat anti-rabbit IgG, (H+L), peroxidase conjugate at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?

  • Immunofluorescent analysis of 4% paraformaldehyde-fixed HepG2 cells labeling Dkk3 with ab186409 at 1/100 dilution, followed by Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody at 1/200 dilution. Counter stained with Dapi (blue).

  • All lanes : Anti-Dkk3 antibody [EPR15611] (ab186409) at 1/5000 dilution

    Lane 1 : Human fetal kidney lysate
    Lane 2 : Human fetal heart lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

    Predicted band size: 38 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?

  • Western blot analysis of Dkk3 in Human fetal brain lysate immunoprecipitated with ab186409 at 1/50 dilution (Lane 1). Lane 2: PBS instead of Human fetal brain lysate.

    Secondary antibody: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

References

This product has been referenced in:
  • Katase N  et al. DKK3 knockdown confers negative effects on the malignant potency of head and neck squamous cell carcinoma cells via the PI3K/Akt and MAPK signaling pathways. Int J Oncol 54:1021-1032 (2019). Read more (PubMed: 30569110) »
  • Leonard JL  et al. The Dkk3 gene encodes a vital intracellular regulator of cell proliferation. PLoS One 12:e0181724 (2017). Read more (PubMed: 28738084) »
See all 4 Publications for this product

Customer reviews and Q&As

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Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - triton-X
Specification
Prostate
Blocking step
1% BSA/5% NHS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formalin

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Submitted Mar 05 2018

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