Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Dkk3 antibody [EPR15611]

Poor
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Prostate)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Sodium Citrate
Permeabilization
Yes - triton-X
Specification
Prostate
Blocking step
1% BSA/5% NHS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formalin

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% BSA/0.3% Triton x-100
Dilution
1/100

Secondary antibody

Dilution
1/200

Additional data

Additional Notes
Tested in human prostate tissue, mouse testes, alongside an optimized primary for CK8 as a positive control for the method and a Secondary only slide as a negative control. No signal at 1:50, 1:100 and 1:500 DKK3 dilutions
Abcam response
We would like to thank this researcher for submitting their review. This antibody had not been tested in IHC and we were unsure of what the results would be. The reviewer received an AbTrial discount code for using the antibody in an untested application.

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Submitted Mar 05 2018

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