Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7867(2)] to DKK4
- Suitable for: WB, Flow Cyt
- Reacts with: Mouse, Rat, Human
Product nameAnti-DKK4 antibody [EPR7867(2)]
See all DKK4 primary antibodies
DescriptionRabbit monoclonal [EPR7867(2)] to DKK4
Tested applicationsSuitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human DKK4 aa 1-100. The exact sequence is proprietary.
Database link: Q9UBT3
- SW480, A375 and Jurkat cell lysates; Jurkat cells.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab172613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa).|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
FunctionAntagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
Tissue specificityExpressed in cerebellum, T-cells, esophagus and lung.
Sequence similaritiesBelongs to the dickkopf family.
DomainThe C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
modificationsAppears to be not glycosylated.
Can be proteolytically processed by a furin-like protease.
- Information by UniProt
- Dickkopf 4 antibody
- Dickkopf gene 4 antibody
- Dickkopf homolog 4 (Xenopus laevis) antibody
All lanes : Anti-DKK4 antibody [EPR7867(2)] (ab172613) at 1/10000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : A375 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 25 kDa
Flow cytometric analysis of permeabilized Jurkat cells labeling DKK4 with ab172613 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
ab172613 has been referenced in 1 publication.
- He S et al. DKK4 enhances resistance to chemotherapeutics 5-Fu and YN968D1 in colorectal cancer cells. Oncol Lett 13:587-592 (2017). WB . PubMed: 28356933