Overview

  • Product name
    Anti-DKK4 antibody [EPR7867(2)]
    See all DKK4 primary antibodies
  • Description
    Rabbit monoclonal [EPR7867(2)] to DKK4
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, Flow Cytmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human DKK4 aa 1-100. The exact sequence is proprietary.
    Database link: Q9UBT3

  • Positive control
    • SW480, A375 and Jurkat cell lysates; Jurkat cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer
    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity
    Tissue culture supernatant
  • Clonality
    Monoclonal
  • Clone number
    EPR7867(2)
  • Isotype
    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab172613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa).
Flow Cyt 1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

 

  • Application notes
    Is unsuitable for ICC/IF,IHC-P or IP.
  • Target

    • Function
      Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
    • Tissue specificity
      Expressed in cerebellum, T-cells, esophagus and lung.
    • Sequence similarities
      Belongs to the dickkopf family.
    • Domain
      The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
    • Post-translational
      modifications
      Appears to be not glycosylated.
      Can be proteolytically processed by a furin-like protease.
    • Cellular localization
      Secreted.
    • Information by UniProt
    • Database links
    • Alternative names
      • Dickkopf 4 antibody
      • Dickkopf gene 4 antibody
      • Dickkopf homolog 4 (Xenopus laevis) antibody
      • Dickkopf homolog 4 antibody
      • Dickkopf related protein 4 antibody
      • Dickkopf-4 antibody
      • Dickkopf-related protein 4 short form antibody
      • Dickkopf4 antibody
      • DKK 4 antibody
      • Dkk-4 antibody
      • DKK4 antibody
      • DKK4_HUMAN antibody
      • hDkk 4 antibody
      • hDkk-4 antibody
      • hDkk4 antibody
      • MGC129562 antibody
      • MGC129563 antibody
      see all

    Images

    • All lanes : Anti-DKK4 antibody [EPR7867(2)] (ab172613) at 1/10000 dilution

      Lane 1 : SW480 cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 25 kDa

    • Flow cytometric analysis of permeabilized Jurkat cells labeling DKK4 with ab172613 at 1/10 dilution (red) or a rabbit IgG (negative) (green).

    References

    This product has been referenced in:
    • He S  et al. DKK4 enhances resistance to chemotherapeutics 5-Fu and YN968D1 in colorectal cancer cells. Oncol Lett 13:587-592 (2017). WB . Read more (PubMed: 28356933) »

    See 1 Publication for this product

    Customer reviews and Q&As

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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