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    dkk4-antibody-epr78672-ab172613.pdf

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Stem Cells Signaling Pathways Wnt Secreted
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RecombinantRabMAb

Recombinant Anti-DKK4 antibody [EPR7867(2)] (ab172613)

  • Datasheet
  • SDS
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Western blot - Anti-DKK4 antibody [EPR7867(2)] (ab172613)
  • Flow Cytometry (Intracellular) - Anti-DKK4 antibody [EPR7867(2)] (ab172613)
  • Anti-DKK4 antibody [EPR7867(2)] (ab172613)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7867(2)] to DKK4
  • Suitable for: Flow Cyt (Intra), WB
  • Reacts with: Human

Conjugates logo Related conjugates and formulations

Carrier Free

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Anti-DKK4 antibody [EPR7867(2)] - BSA and Azide free (ab249708)

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Overview

  • Product name

    Anti-DKK4 antibody [EPR7867(2)]
    See all DKK4 primary antibodies
  • Description

    Rabbit monoclonal [EPR7867(2)] to DKK4
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), WBmore details
    Unsuitable for: ICC/IF,IHC-P or IP
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide within Human DKK4 aa 1-100. The exact sequence is proprietary.
    Database link: Q9UBT3

  • Positive control

    • SW480, A375 and Jurkat cell lysates; Jurkat cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7867(2)
  • Isotype

    IgG
  • Research areas

    • Stem Cells
    • Signaling Pathways
    • Wnt
    • Secreted

Associated products

  • Alternative Versions

    • Anti-DKK4 antibody [EPR7867(2)] - BSA and Azide free (ab249708)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Related Products

    • SW480 whole cell lysate (ab3957)
    • Jurkat whole cell lysate (ab7899)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab172613 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa).
Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

WB
1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa).
Application notes
Is unsuitable for ICC/IF,IHC-P or IP.

Target

  • Function

    Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease.
  • Tissue specificity

    Expressed in cerebellum, T-cells, esophagus and lung.
  • Sequence similarities

    Belongs to the dickkopf family.
  • Domain

    The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6.
  • Post-translational
    modifications

    Appears to be not glycosylated.
    Can be proteolytically processed by a furin-like protease.
  • Cellular localization

    Secreted.
  • Target information above from: UniProt accession Q9UBT3 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 27121 Human
    • Entrez Gene: 234130 Mouse
    • Entrez Gene: 502097 Rat
    • GenBank: NP_055235.1 Human
    • Omim: 605417 Human
    • SwissProt: Q9UBT3 Human
    • SwissProt: Q8VEJ3 Mouse
    • Unigene: 159311 Human
    • Alternative names

      • Dickkopf 4 antibody
      • Dickkopf gene 4 antibody
      • Dickkopf homolog 4 (Xenopus laevis) antibody
      • Dickkopf homolog 4 antibody
      • Dickkopf related protein 4 antibody
      • Dickkopf-4 antibody
      • Dickkopf-related protein 4 short form antibody
      • Dickkopf4 antibody
      • DKK 4 antibody
      • Dkk-4 antibody
      • DKK4 antibody
      • DKK4_HUMAN antibody
      • hDkk 4 antibody
      • hDkk-4 antibody
      • hDkk4 antibody
      • MGC129562 antibody
      • MGC129563 antibody
      see all

    Images

    • Western blot - Anti-DKK4 antibody [EPR7867(2)] (ab172613)
      Western blot - Anti-DKK4 antibody [EPR7867(2)] (ab172613)
      All lanes : Anti-DKK4 antibody [EPR7867(2)] (ab172613) at 1/10000 dilution

      Lane 1 : SW480 cell lysate
      Lane 2 : A375 cell lysate
      Lane 3 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 25 kDa

    • Flow Cytometry (Intracellular) - Anti-DKK4 antibody [EPR7867(2)] (ab172613)
      Flow Cytometry (Intracellular) - Anti-DKK4 antibody [EPR7867(2)] (ab172613)

      Intracellular flow cytometric analysis of permeabilized Jurkat cells labeling DKK4 with ab172613 at 1/10 dilution (red) or arabbit IgG (negative) (green). 

       

       

    • Anti-DKK4 antibody [EPR7867(2)] (ab172613)
      Anti-DKK4 antibody [EPR7867(2)] (ab172613)

    Protocols

    • Western blot protocols
    • Flow cytometry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab172613? Please let us know so that we can cite the reference in this datasheet.

    ab172613 has been referenced in 1 publication.

    • He S  et al. DKK4 enhances resistance to chemotherapeutics 5-Fu and YN968D1 in colorectal cancer cells. Oncol Lett 13:587-592 (2017). WB . PubMed: 28356933

    Customer reviews and Q&As

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