Recombinant Anti-DKK4 antibody [EPR7867(2)] (ab172613)
- Datasheet
- References (1)
- Protocols
Overview
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Product name
Anti-DKK4 antibody [EPR7867(2)]
See all DKK4 primary antibodies -
Description
Rabbit monoclonal [EPR7867(2)] to DKK4 -
Host species
Rabbit -
Tested applications
Suitable for: WB, Flow Cytmore details
Unsuitable for: ICC/IF,IHC-P or IP -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide within Human DKK4 aa 1-100. The exact sequence is proprietary.
Database link: Q9UBT3 -
Positive control
- SW480, A375 and Jurkat cell lysates; Jurkat cells.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant -
Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
EPR7867(2) -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab172613 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000 - 1/5000. Detects a band of approximately 40 kDa (predicted molecular weight: 25 kDa). | |
| Flow Cyt | 1/10 - 1/100. ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
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Target
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Function
Antagonizes canonical Wnt signaling by inhibiting LRP5/6 interaction with Wnt and by forming a ternary complex with the transmembrane protein KREMEN that promotes internalization of LRP5/6. DKKs play an important role in vertebrate development, where they locally inhibit Wnt regulated processes such as antero-posterior axial patterning, limb development, somitogenesis and eye formation. In the adult, Dkks are implicated in bone formation and bone disease, cancer and Alzheimer disease. -
Tissue specificity
Expressed in cerebellum, T-cells, esophagus and lung. -
Sequence similarities
Belongs to the dickkopf family. -
Domain
The C-terminal cysteine-rich domain mediates interaction with LRP5 and LRP6. -
Post-translational
modificationsAppears to be not glycosylated.
Can be proteolytically processed by a furin-like protease. -
Cellular localization
Secreted. - Information by UniProt
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Database links
- Entrez Gene: 27121 Human
- Entrez Gene: 234130 Mouse
- Entrez Gene: 502097 Rat
- GenBank: NP_055235.1 Human
- Omim: 605417 Human
- SwissProt: Q9UBT3 Human
- SwissProt: Q8VEJ3 Mouse
- Unigene: 159311 Human
see all -
Alternative names
- Dickkopf 4 antibody
- Dickkopf gene 4 antibody
- Dickkopf homolog 4 (Xenopus laevis) antibody
see all
Images
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![Western blot - Anti-DKK4 antibody [EPR7867(2)] (ab172613)](https://www.abcam.com/ps/products/172/ab172613/Images/ab172613-195611-anti-dkk4-antibody-epr78672-western-blot.jpg)
Western blot - Anti-DKK4 antibody [EPR7867(2)] (ab172613)All lanes : Anti-DKK4 antibody [EPR7867(2)] (ab172613) at 1/10000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : A375 cell lysate
Lane 3 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 25 kDa -
![Flow Cytometry - Anti-DKK4 antibody [EPR7867(2)] (ab172613)](https://www.abcam.com/ps/products/172/ab172613/Images/ab172613-195612-anti-dkk4-antibody-epr78672-flow-cytometry.jpg)
Flow Cytometry - Anti-DKK4 antibody [EPR7867(2)] (ab172613)Flow cytometric analysis of permeabilized Jurkat cells labeling DKK4 with ab172613 at 1/10 dilution (red) or a rabbit IgG (negative) (green).
Datasheets and documents
References
This product has been referenced in:
- He S et al. DKK4 enhances resistance to chemotherapeutics 5-Fu and YN968D1 in colorectal cancer cells. Oncol Lett 13:587-592 (2017). WB . Read more (PubMed: 28356933) »
