Product nameAnti-DLC-A antibody [EPR11240]
See all DLC-A primary antibodies
DescriptionRabbit monoclonal [EPR11240] to DLC-A
Tested applicationsSuitable for: WB, IHC-P, ICC/IF, IPmore details
Unsuitable for: Flow Cyt
Species reactivityReacts with: Mouse, Rat, Human
Recombinant fragment corresponding to Human DLC-A.
Database link: Q9Y6G9
- K562, HeLa, fetal brain, fetal heart and Jurkat lysates; Human heart and pancreas tissues; K562 cells; Immunoprecipitation pellet from fetal brain lysate.
Storage instructionsShipped at 4°C. Store at -20ºC.
Storage bufferPreservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
PurityTissue culture supernatant
Our Abpromise guarantee covers the use of ab157468 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Predicted molecular weight: 57 kDa.|
|IHC-P||1/100 - 1/250.|
|ICC/IF||1/100 - 1/250.|
|IP||1/10 - 1/100.|
FunctionActs as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores.
Sequence similaritiesBelongs to the dynein light intermediate chain family.
modificationsPhosphorylated during mitosis but not in interphase.
Cellular localizationCytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole.
- Information by UniProt
- Cytoplasmic dynein 1 light intermediate chain 1 antibody
- cytosolic antibody
- DC1L1_HUMAN antibody
All lanes : Anti-DLC-A antibody [EPR11240] (ab157468) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : Fetal brain lysate
Lane 4 : Fetal heart lysate
Lane 5 : Jurkat cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 57 kDa
Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling DLC-A with ab157468 at 1/100 dilution.
Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DLC-A with ab157468 at 1/100 dilution.
Immunofluorescent analysis of K562 cells labeling DLC-A with ab157468 at 1/100 dilution.
Anti-DLC-A antibody [EPR11240] (ab157468) at 1/1000 dilution + immunoprecipitation pellet from fetal brain lysate
HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.
Predicted band size: 57 kDa
This product has been referenced in:
- Neubauer HA et al. Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma. Oncogene 38:1151-1165 (2019). Read more (PubMed: 30250299) »
- Itoh Y et al. PDK1-Akt pathway regulates radial neuronal migration and microtubules in the developing mouse neocortex. Proc Natl Acad Sci U S A 113:E2955-64 (2016). Read more (PubMed: 27170189) »