Recombinant
RabMAb

Recombinant Anti-DLC-A antibody [EPR11240] (ab157468)

Overview

  • Product name

    Anti-DLC-A antibody [EPR11240]
    See all DLC-A primary antibodies
  • Description

    Rabbit monoclonal [EPR11240] to DLC-A
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, IPmore details
    Unsuitable for: Flow Cyt
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to Human DLC-A.
    Database link: Q9Y6G9

  • Positive control

    • K562, HeLa, fetal brain, fetal heart and Jurkat lysates; Human heart and pancreas tissues; K562 cells; Immunoprecipitation pellet from fetal brain lysate.
  • General notes

    Previously labelled as Cytoplasmic dynein 1 light intermediate chain 1.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR11240
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab157468 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 57 kDa.
IHC-P 1/100 - 1/250.
ICC/IF 1/100 - 1/250.
IP 1/10 - 1/100.
  • Application notes
    Is unsuitable for Flow Cyt.
  • Target

    • Function

      Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores.
    • Sequence similarities

      Belongs to the dynein light intermediate chain family.
    • Post-translational
      modifications

      Phosphorylated during mitosis but not in interphase.
    • Cellular localization

      Cytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole.
    • Information by UniProt
    • Database links

    • Alternative names

      • Cytoplasmic dynein 1 light intermediate chain 1 antibody
      • cytosolic antibody
      • DC1L1_HUMAN antibody
      • DLC-A antibody
      • DNCLI1 antibody
      • dync1li1 antibody
      • Dynein cytoplasmic 1 light intermediate chain 1 antibody
      • Dynein cytoplasmic light intermediate polypeptide 1 antibody
      • Dynein light chain A antibody
      • Dynein light intermediate chain 1 antibody
      • Dynein light intermediate chain 1 cytosolic antibody
      • FLJ10219 antibody
      • LIC1 antibody
      see all

    Images

    • All lanes : Anti-DLC-A antibody [EPR11240] (ab157468) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : HeLa cell lysate
      Lane 3 : Fetal brain lysate
      Lane 4 : Fetal heart lysate
      Lane 5 : Jurkat cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 57 kDa

    • Immunohistochemical analysis of paraffin-embedded Human heart tissue labeling DLC-A with ab157468 at 1/100 dilution.

    • Immunohistochemical analysis of paraffin-embedded Human pancreas tissue labeling DLC-A with ab157468 at 1/100 dilution.

    • Immunofluorescent analysis of K562 cells labeling DLC-A with ab157468 at 1/100 dilution.

    • Anti-DLC-A antibody [EPR11240] (ab157468) at 1/1000 dilution + immunoprecipitation pellet from fetal brain lysate

      Secondary
      HRP-conjugated anti-rabbit IgG, which preferentially detects the non-reduced form of rabbit IgG.

      Predicted band size: 57 kDa

    References

    This product has been referenced in:

    • Neubauer HA  et al. Cytoplasmic dynein regulates the subcellular localization of sphingosine kinase 2 to elicit tumor-suppressive functions in glioblastoma. Oncogene 38:1151-1165 (2019). Read more (PubMed: 30250299) »
    • Itoh Y  et al. PDK1-Akt pathway regulates radial neuronal migration and microtubules in the developing mouse neocortex. Proc Natl Acad Sci U S A 113:E2955-64 (2016). Read more (PubMed: 27170189) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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