Key features and details
- Rabbit polyclonal to DLC-A - N-terminal
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Product nameAnti-DLC-A antibody - N-terminal
See all DLC-A primary antibodies
DescriptionRabbit polyclonal to DLC-A - N-terminal
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Guinea pig, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
- HeLa, 293T, and Jurkat whole cell lysates.
Previously labelled as Cytoplasmic dynein 1 light intermediate chain 1.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH 7 to 8
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab185994 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 57 kDa.|
|IP||Use at 2-10 µg/mg of lysate.|
FunctionActs as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores.
Sequence similaritiesBelongs to the dynein light intermediate chain family.
modificationsPhosphorylated during mitosis but not in interphase.
Cellular localizationCytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole.
- Information by UniProt
- Cytoplasmic dynein 1 light intermediate chain 1 antibody
- cytosolic antibody
- DC1L1_HUMAN antibody
All lanes : Anti-DLC-A antibody - N-terminal (ab185994) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate
Lane 2 : 293T whole cell lysate
Lane 3 : Jurkat whole cell lysate
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 57 kDa
Exposure time: 3 minutes
Immunoprecipitation. ab185994 at 0.1 µg/ml staining DLC-A in 293T whole cell lysate immunoprecipitated using ab185994 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Lane 2: controle IgG.
ab185994 has not yet been referenced specifically in any publications.