Anti-DLC-A antibody - N-terminal (ab185994)

Overview

  • Product name

    Anti-DLC-A antibody - N-terminal
    See all DLC-A primary antibodies
  • Description

    Rabbit polyclonal to DLC-A - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IPmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Guinea pig, Cow, Cat, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan
  • Immunogen

    Synthetic peptide within Human DLC-A aa 1-50 (N terminal). The exact sequence is proprietary. NP_057225.2
    Sequence:

    MAAVGRVGSF GSSPPGLSST YTGGPLGNEI ASGNGGAAAG DDEDGQNLWS


    Database link: Q9Y6G9

  • Positive control

    • HeLa, 293T, and Jurkat whole cell lysates.
  • General notes

    Previously labelled as Cytoplasmic dynein 1 light intermediate chain 1. 

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservative: 0.09% Sodium azide
    Constituent: 99% Tris citrate/phosphate

    pH 7 to 8
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG

Applications

Our Abpromise guarantee covers the use of ab185994 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000 - 1/10000. Predicted molecular weight: 57 kDa.
IP Use at 2-10 µg/mg of lysate.

Target

  • Function

    Acts as one of several non-catalytic accessory components of the cytoplasmic dynein 1 complex that are thought to be involved in linking dynein to cargos and to adapter proteins that regulate dynein function. Cytoplasmic dynein 1 acts as a motor for the intracellular retrograde motility of vesicles and organelles along microtubules. May play a role in binding dynein to membranous organelles or chromosomes. Probably involved in the microtubule-dependent transport of pericentrin. Is required for progress throuh the spindle assembly checkpoint. The phosphorylated form appears to be involved in the selective removal of MAD1L1 and MAD1L2 but not BUB1B from kinetochores.
  • Sequence similarities

    Belongs to the dynein light intermediate chain family.
  • Post-translational
    modifications

    Phosphorylated during mitosis but not in interphase.
  • Cellular localization

    Cytoplasm. Chromosome > centromere > kinetochore. Cytoplasm > cytoskeleton > spindle pole.
  • Information by UniProt
  • Database links

  • Alternative names

    • Cytoplasmic dynein 1 light intermediate chain 1 antibody
    • cytosolic antibody
    • DC1L1_HUMAN antibody
    • DLC-A antibody
    • DNCLI1 antibody
    • dync1li1 antibody
    • Dynein cytoplasmic 1 light intermediate chain 1 antibody
    • Dynein cytoplasmic light intermediate polypeptide 1 antibody
    • Dynein light chain A antibody
    • Dynein light intermediate chain 1 antibody
    • Dynein light intermediate chain 1 cytosolic antibody
    • FLJ10219 antibody
    • LIC1 antibody
    see all

Images

  • All lanes : Anti-DLC-A antibody - N-terminal (ab185994) at 0.1 µg/ml

    Lane 1 : HeLa whole cell lysate
    Lane 2 : 293T whole cell lysate
    Lane 3 : Jurkat whole cell lysate

    Lysates/proteins at 50 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 57 kDa


    Exposure time: 3 minutes
  • Immunoprecipitation. ab185994 at 0.1 µg/ml staining DLC-A in 293T whole cell lysate immunoprecipitated using ab185994 at 6 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
    Lane 2: controle IgG.

References

ab185994 has not yet been referenced specifically in any publications.

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