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    dlc1-antibody-ab126257.pdf

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Tags & Cell Markers Cell Type Markers Tumor Associated
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Anti-DLC1 antibody (ab126257)

  • Datasheet
  • SDS
Submit a review Submit a question References (3)

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Western blot - Anti-DLC1 antibody (ab126257)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC1 antibody (ab126257)

Key features and details

  • Rabbit polyclonal to DLC1
  • Suitable for: WB, IHC-P
  • Reacts with: Human
  • Isotype: IgG

You may also be interested in

Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-DLC1 antibody
    See all DLC1 primary antibodies
  • Description

    Rabbit polyclonal to DLC1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant fragment, corresponding to a region within C terminal amino acids 1271 -1516 of Human DLC1 (Q96QB1).

  • Positive control

    • A549 and HepG2 cell lysates and Human Breast ca tissue.
  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.01% Thimerosal (merthiolate)
    Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Cell Type Markers
    • Tumor Associated
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Cancer susceptibility
    • Tumor Suppressors
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • Hep G2 cytoplasmic extract lysate (ab14659)
    • A549 whole cell lysate (ab7910)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab126257 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/500 - 1/3000. Predicted molecular weight: 171 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Notes
WB
1/500 - 1/3000. Predicted molecular weight: 171 kDa.
IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

  • Function

    Functions as a GTPase-activating protein specific for Rho and an activator of PLCD1 in vivo and induces morphological changes and detachment through cytoskeletal reorganization.
  • Tissue specificity

    Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus.
  • Sequence similarities

    Contains 1 Rho-GAP domain.
    Contains 1 SAM (sterile alpha motif) domain.
    Contains 1 START domain.
  • Cellular localization

    Cytoplasm.
  • Target information above from: UniProt accession Q96QB1 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 10395 Human
    • Omim: 604258 Human
    • SwissProt: Q96QB1 Human
    • Unigene: 134296 Human
    • Alternative names

      • ARHGAP 7 antibody
      • ARHGAP7 antibody
      • Deleted in liver cancer 1 antibody
      • Deleted in liver cancer 1 protein antibody
      • DLC 1 antibody
      • Dlc-1 antibody
      • DLC1 antibody
      • DLC1 Rho GTPase activating protein antibody
      • FLJ21120 antibody
      • HP antibody
      • HP protein antibody
      • KIAA1723 antibody
      • p122 RhoGAP antibody
      • RHG07_HUMAN antibody
      • Rho GTPase activating protein 7 antibody
      • Rho GTPase-activating protein 7 antibody
      • Rho type GTPase activating protein 7 antibody
      • Rho-type GTPase-activating protein 7 antibody
      • StAR related lipid transfer (START) domain containing 12 antibody
      • StAR related lipid transfer protein 12 antibody
      • StAR-related lipid transfer protein 12 antibody
      • STARD 12 antibody
      • StARD12 antibody
      • START domain containing protein 12 antibody
      • START domain-containing protein 12 antibody
      see all

    Images

    • Western blot - Anti-DLC1 antibody (ab126257)
      Western blot - Anti-DLC1 antibody (ab126257)
      Anti-DLC1 antibody (ab126257) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

      Predicted band size: 171 kDa



      Gel concentration: 5%
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC1 antibody (ab126257)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC1 antibody (ab126257)
      ab126257, at 1/500, staining DLC1 in Human Breast ca tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P).

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (3)

    Publishing research using ab126257? Please let us know so that we can cite the reference in this datasheet.

    ab126257 has been referenced in 3 publications.

    • Sun L  et al. High expression of DLC family proteins predicts better prognosis and inhibits tumor progression in NSCLC. Mol Med Rep 19:4881-4889 (2019). PubMed: 31059015
    • Ye Y  et al. Exosomal miR-141-3p regulates osteoblast activity to promote the osteoblastic metastasis of prostate cancer. Oncotarget 8:94834-94849 (2017). PubMed: 29212270
    • Xiao P  et al. miR-429 promotes the proliferation of non-small cell lung cancer cells via targeting DLC-1. Oncol Lett 12:2163-2168 (2016). WB ; Human . PubMed: 27602157

    Customer reviews and Q&As

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