Anti-DLC1 antibody (ab126257)
Key features and details
- Rabbit polyclonal to DLC1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-DLC1 antibody
See all DLC1 primary antibodies -
Description
Rabbit polyclonal to DLC1 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Recombinant fragment, corresponding to a region within C terminal amino acids 1271 -1516 of Human DLC1 (Q96QB1).
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Positive control
- A549 and HepG2 cell lysates and Human Breast ca tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 78.99% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab126257 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 171 kDa.
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| IHC-P |
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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| Notes |
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WB
1/500 - 1/3000. Predicted molecular weight: 171 kDa. |
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IHC-P
1/100 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Functions as a GTPase-activating protein specific for Rho and an activator of PLCD1 in vivo and induces morphological changes and detachment through cytoskeletal reorganization. -
Tissue specificity
Highest level of expression in the spleen, with rather lower levels in prostate, testis, ovary, small intestine and colon, but none in the thymus. -
Sequence similarities
Contains 1 Rho-GAP domain.
Contains 1 SAM (sterile alpha motif) domain.
Contains 1 START domain. -
Cellular localization
Cytoplasm. - Information by UniProt
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Database links
- Entrez Gene: 10395 Human
- Omim: 604258 Human
- SwissProt: Q96QB1 Human
- Unigene: 134296 Human
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Alternative names
- ARHGAP 7 antibody
- ARHGAP7 antibody
- Deleted in liver cancer 1 antibody
see all
Images
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Western blot - Anti-DLC1 antibody (ab126257)Anti-DLC1 antibody (ab126257) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 171 kDa
Gel concentration: 5% -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-DLC1 antibody (ab126257)ab126257, at 1/500, staining DLC1 in Human Breast ca tissue by immunohistochemistry [Paraffin Embedded Tissues (IHC-P).
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab126257 has been referenced in 3 publications.
- Sun L et al. High expression of DLC family proteins predicts better prognosis and inhibits tumor progression in NSCLC. Mol Med Rep 19:4881-4889 (2019). PubMed: 31059015
- Ye Y et al. Exosomal miR-141-3p regulates osteoblast activity to promote the osteoblastic metastasis of prostate cancer. Oncotarget 8:94834-94849 (2017). PubMed: 29212270
- Xiao P et al. miR-429 promotes the proliferation of non-small cell lung cancer cells via targeting DLC-1. Oncol Lett 12:2163-2168 (2016). WB ; Human . PubMed: 27602157