Anti-DLG5 antibody (ab231283)
Key features and details
- Rabbit polyclonal to DLG5
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-DLG5 antibody
See all DLG5 primary antibodies -
Description
Rabbit polyclonal to DLG5 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment
Predicted to work with: Rat -
Immunogen
Recombinant fragment (His-T7-tag) corresponding to Mouse DLG5 aa 1700 to the C-terminus. (Expressed in E.coli)
Database link: E9Q9R9 -
Positive control
- IHC-P: Mouse brain tissue. WB: Recombinant mouse DLG5 protein; PC-3 cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab231283 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231283 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 214 kDa.
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Notes |
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IHC-P
Use a concentration of 5 - 20 µg/ml. |
WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 214 kDa. |
Target
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Function
May play a role at the plasma membrane in the maintenance of the structure of epithelial cells and in the transmission of extracellular signals to the membrane and cytoskeleton. -
Tissue specificity
Highly expressed in the placenta and prostate. Expressed at a lower level in the thyroid, spinal cord, trachea, adrenal gland, skeletal muscle, pancreas, heart, brain, liver and kidney. A short splice product shows more limited expression, being absent from at least the brain. -
Sequence similarities
Belongs to the MAGUK family.
Contains 1 CARD domain.
Contains 1 guanylate kinase-like domain.
Contains 4 PDZ (DHR) domains.
Contains 1 SH3 domain. -
Cellular localization
Cell junction. Cell membrane. Localized at sites of cell-cell contact. - Information by UniProt
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Database links
- Entrez Gene: 9231 Human
- Entrez Gene: 71228 Mouse
- Entrez Gene: 305645 Rat
- Omim: 604090 Human
- SwissProt: Q8TDM6 Human
- Unigene: 652690 Human
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Alternative names
- Discs large homolog 5 (Drosophila) antibody
- Discs large homolog 5 antibody
- Discs large protein LP DLG antibody
see all
Images
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Anti-DLG5 antibody (ab231283) at 2 µg/ml + PC-3 (human prostate adenocarcinoma cell line) cell lysate.
Predicted band size: 214 kDa -
Anti-DLG5 antibody (ab231283) at 2 µg/ml + Recombinant mouse DLG5 protein.
Predicted band size: 214 kDa -
Formalin-fixed, paraffin-embedded mouse brain tissue stained for DLG5 using ab231283 at 20 µg/ml in immunohistochemical analysis.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (1)
ab231283 has been referenced in 1 publication.
- Bai W et al. LINC00589-dominated ceRNA networks regulate multiple chemoresistance and cancer stem cell-like properties in HER2+ breast cancer. NPJ Breast Cancer 8:115 (2022). PubMed: 36309503